HHATL antibody (N-Term)

Catalog No. ABIN1450142

The Rabbit Polyclonal anti-HHATL antibody is suitable to detect HHATL in samples from Human, Mouse and Rat. It has been validated for WB, IHC (p), IF and EIA.

Quick Overview for HHATL antibody (N-Term) (ABIN1450142)

Target: HHATL (Hedgehog Acyltransferase-Like (HHATL))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HHATL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-HHATL Antibody

Binding Specificity: N-Term

Purification: Affinity chromatography purified via peptide column

Immunogen: 19 amino acid synthetic peptide near the amino terminus of Human HHATL

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for HHATL

Target: HHATL (Hedgehog Acyltransferase-Like (HHATL))

Alternative Name: GUP1 / HHATL

Background: The Protein-cysteine N-palmitoyltransferase HHAT-like protein (HHATL), also known at the mammalian glycerol uptake/transporter 1 (Gup1) is a homolog of the S. cerevisiae Gup1. It is an endoplasmic reticulum (ER) membrane protein that co-localizes with the mammalian hedgehog acyltransferse Skn and interferes with its N-terminal palmitoylation of Sonic hedgehog (SHH), suggesting that HHATL acts as a negative regulator for N-terminal palmitoylation of SHH. HHATL is highly expressed in normal skin, but becomes down-regulated in skin squamous cell carcinoma, suggesting it may play a role in its development .Synonyms: C3orf3, Glycerol uptake/transporter homolog, Hedgehog acyltransferase-like protein, KIAA1173, Protein-cysteine N-palmitoyltransferase HHAT-like protein

Gene ID: 57467

NCBI Accession: NP_065758

Pathways: Hedgehog Signaling