SPAM1 antibody (AA 100-300)

Catalog No. ABIN1513554

The Rabbit Polyclonal anti-SPAM1 antibody (ABIN1513554) specifically detects SPAM1 in WB, IHC and IF. The antibody is reactive with Human samples.

Quick Overview for SPAM1 antibody (AA 100-300) (ABIN1513554)

Target: SPAM1 (Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPAM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SPAM1 Antibody

Binding Specificity: AA 100-300

Sequence: PYIDSITGVT VNGGIPQKIS LQDHLDKAKK DITFYMPVDN LGMAVIDWEE WRPTWARNWK PKDVYKNRSI ELVQQQNVQL SLTEATEKAK QEFEKAGKDF LVETIKLGKL LRPNHLWGYY LFPDCYNHHY KKPGYNGSCF NVEIKRNDDL SWLWNESTAL YPSIYLNTQQ SPVAATLYVR NRVREAIRVS KIPDAKSPLP V

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human SPAM1 (NP_003108.2).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for SPAM1

Target: SPAM1 (Sperm Adhesion Molecule 1 (PH-20 Hyaluronidase, Zona Pellucida Binding) (SPAM1))

Alternative Name: SPAM1

Background: Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1, another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene.,SPAM1,HEL-S-96n,HYA1,HYAL1,HYAL3,HYAL5,PH-20,PH20,SPAG15,Cancer,Invasion and Metastasis,Signal Transduction,Cell Biology & Developmental Biology,Cytoskeleton,Extracellular Matrix,SPAM1

Molecular Weight: 57 kDa/58 kDa

Gene ID: 6677

UniProt: P38567