ATPase antibody (pSer16)

Catalog No. ABIN1531300

The Rabbit Polyclonal anti-ATPase antibody is suitable to detect ATPase in samples from Human, Mouse and Rat. It has been validated for ELISA, WB and IHC.

Quick Overview for ATPase antibody (pSer16) (ABIN1531300)

Target: ATPase (DNAH8) (Dynein, Axonemal, Heavy Chain 8 (DNAH8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATPase antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ATPase Antibody

Binding Specificity: AA 5-54, pSer16

Specificity:

ATPase (Phospho-Ser16) Antibody detects endogenous levels of ATPase only when phosphorylated at Ser16.

PhosphorylationH:S16 M:S16 R:S16

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human ATPase around the phosphorylation site of Ser16.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

Comment:

Unigene-Number: Hs.371889 (NCBI Gene Symbol: ATP1A1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for ATPase

Target: ATPase (DNAH8) (Dynein, Axonemal, Heavy Chain 8 (DNAH8))

Alternative Name: ATPase

Background:

Synonyms: A1A1, AT1A1, ATPase, Na+/K+ transporting, alpha 1 polypeptide, Hypothetical protein MGC38419, Na+/K+ ATPase 1, Na,K-ATPase 1, Sodium pump 1, Sodium/potassium-transporting ATPase alpha-1 chain precursor

NCBI Gene Symbol: ATP1A1

Molecular Weight: 112 kDa

Gene ID: 476

OMIM: 182310

UniProt: P05023

Pathways: Synaptic Membrane, Proton Transport