ABL1 antibody (pTyr393, pTyr412)

Catalog No. ABIN1531765

This Rabbit Polyclonal antibody specifically detects ABL1 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for ABL1 antibody (pTyr393, pTyr412) (ABIN1531765)

Target: ABL1 (C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-ABL1 Antibody

Binding Specificity: AA 406-455, pTyr393, pTyr412

Purpose: Abl1/2 (phospho Tyr393/439) Antibody

Specificity: Phospho-Abl1/2 (Y393/439) Polyclonal Antibody detects endogenous levels of Abl1/2 protein only when phosphorylated at Y393/439.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Abl around the phosphorylation site of Tyr393/412. AA range:406-455

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for ABL1

Target: ABL1 (C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1))

Alternative Name: Abl

Background:

Synonyms: ABL1; ABL; JTK7; Tyrosine-protein kinase ABL1; Abelson murine leukemia viral oncogene homolog 1; Abelson tyrosine-protein kinase 1; Proto-oncogene c-Abl; p150; ABL2; ABLL; ARG; Abelson tyrosine-protein kinase 2; Abelson murine leukemia vira

Background: This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons. [pr

Gene Name: ABL1/ABL2

Protein Name: Tyrosine-protein kinase ABL1/2

Molecular Weight: 55 kDa

Gene ID: 25, 27

OMIM: 189980

UniProt: P00519

Pathways: Apoptosis, Regulation of Muscle Cell Differentiation, Platelet-derived growth Factor Receptor Signaling, Lipid Metabolism