SAE1 antibody (AA 221-270)

Catalog No. ABIN1533423

This Rabbit Polyclonal antibody specifically detects SAE1 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SAE1 antibody (AA 221-270) (ABIN1533423)

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SAE1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SAE1 Antibody

Binding Specificity: AA 221-270

Purpose: SAE1 Antibody

Specificity: SAE1 Polyclonal Antibody detects endogenous levels of SAE1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human AOS1. AA range:221-270

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for SAE1

Target: SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))

Alternative Name: AOS1

Background:

Synonyms: SAE1, AOS1, SUA1, UBLE1A, SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A

Background: SUMO1 activating enzyme subunit 1(SAE1) Homo sapiens Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1, MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010],

Gene Name: SAE1

Protein Name: SUMO-activating enzyme subunit 1

Molecular Weight: 38 kDa

Gene ID: 10055

OMIM: 613294

UniProt: Q9UBE0