FASL antibody

Catalog No. ABIN155064

The Mouse Monoclonal anti-FASL antibody (Clone I-6060) (ABIN155064) specifically detects FASL in WB, IP, IHC (p) and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for FASL antibody (ABIN155064)

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FASL antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: I-6060

Product Details anti-FASL Antibody

Specificity: This antibody detects Fas Ligand.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: FASLG, APT1LG1, FASL, TNFSF6, CD95L protein, APTL, Tumor necrosis factor ligandsuperfamily member 6, Fas antigen ligand, Apoptosis antigen ligand

Purification: Affiniry Chromatography on Protein G

Immunogen: Recombinant Human Fas Ligand.

Isotype: IgG1

Application Details

Application Notes: Western Blot (1-4 μg/mL): HeLa cell lysate can be used as a Positive Control and a 40 kDaband should be detected. Immunoprecipitation. Immunohistochemistry on Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, 0.02 % Thimerosal, 50 % Glycerol, 1 % BSA

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -20 °C -70 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for FASL

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Alternative Name: CD178 / Fas Ligand

Background: Fas (APO-1, CD95) is a 45 kDa cell surface protein that mediates apoptosis when crosslinked with agonistic anti-Fas antibodies or Fas ligand (FasL). FasL is a tumor necrosis factor (TNF)-related type II membrane protein and is expressed in various tissue and cells. Both Fas and FasL are thought to play an important role in the apoptosis processes that takes place during T cell development.Synonyms: APT1LG1, APTL, Apoptosis antigen ligand, CD95L protein, FASL, FASLG, Fas antigen ligand, TNFSF6, Tumor necrosis factor ligand superfamily member 6

Gene ID: 356

UniProt: P48023

Pathways: Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity