TUBB antibody

Catalog No. ABIN1574254

Product Details anti-TUBB Antibody

Target: TUBB (Tubulin, beta (TUBB))

Reactivity: Human, Mouse, Rat, Rabbit, Pig, Chicken, Cow, Fish, Goat

Host: Mouse

Clonality: Monoclonal

Conjugate: This TUBB antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Specificity: Beta-tubulin Antibody (2G7D4), mAb, Mouse reacts with human, monkey, goat, bovine, swine, rabbit, rat, mouse, chicken and fish beta-tubulin. This product has not yet been tested in other species.

Cross-Reactivity (Details): beta-tubulin Antibody (2G7D4), mAb, Mouse reacts with human, monkey, goat, bovine, swine, rabbit, rat, mouse, chicken and fish beta-tubulin. This product has not yet been tested in other species.

Purification: Protein A affinity column

Immunogen: purified human recombinant full-length β-tubulin protein

Clone: 2G7D4

Isotype: IgG1

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.2-1 µg/mL Flow cytometry:1 µg for 1 x 106 cells

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

References for anti-TUBB antibody (ABIN1574254)

Fisher, Resnick, De, Acevedo, Lu, Schroeder, Nicholson: "Cyclic cis-Locked Phospho-Dipeptides Reduce Entry of AβPP into Amyloidogenic Processing Pathway." in: Journal of Alzheimer's disease : JAD, Vol. 55, Issue 1, pp. 391-410, (2016) (PubMed).

Huang, Nishi, Li, Ho, Dong, Chang, Chang: "Acyl-coenzyme A:cholesterol acyltransferase 1 - significance of single-nucleotide polymorphism at residue 526 and the role of Pro347 near the fifth transmembrane domain." in: The FEBS journal, Vol. 281, Issue 7, pp. 1773-83, (2014) (PubMed).

Sukthankar, Avila, Whitaker, Iwamoto, Morgenstern, Apostolidis, Liu, Hanzlik, Dadachova, Tomich: "Branched amphiphilic peptide capsules: cellular uptake and retention of encapsulated solutes." in: Biochimica et biophysica acta, Vol. 1838, Issue 9, pp. 2296-305, (2014) (PubMed).

Infanger, Cho, Lopez, Mohanan, Liu, Gursel, Boockvar, Fischbach: "Glioblastoma stem cells are regulated by interleukin-8 signaling in a tumoral perivascular niche." in: Cancer research, Vol. 73, Issue 23, pp. 7079-89, (2013) (PubMed).

Wang, Chatterjee, Jeon, Akerman, Vander Heiden, Cantley, Krainer: "Exon-centric regulation of pyruvate kinase M alternative splicing via mutually exclusive exons." in: Journal of molecular cell biology, Vol. 4, Issue 2, pp. 79-87, (2012) (PubMed).

Target Details for TUBB

Target: TUBB (Tubulin, beta (TUBB))

Alternative Name: beta-Tubulin (TUBB Products)

Background: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. It is a dimer of alpha (alpha) and beta (beta) chains. beta-tubulin is ubiquitously expressed with highest levels in spleen, thymus and immature brain and it has a highly acidic C-terminal region which may bind cations such as calcium. This antibody can be used as a loading control on Western blots. beta-tubulin Antibody (2G7D4), mAb, Mouse is produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes obtained from mouse immunized with purified human recombinant full-length beta-tubulin protein.

Pathways: Microtubule Dynamics, M Phase