Small Subunit H of Psii (Psbh) antibody

Antigen: Small Subunit H of Psii (Psbh)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Balsam fir (Abies balsamea), Arabidopsis thaliana, Barley (Hordeum vulgare), Eastern White Pine (Pinus strobus), Spinach (Spinacia oleracea), Synechococcus PCC 7942

Application: Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN173362

Additional Information

Immunogen: KLH-conjugated synthetic peptide chosen from PsbH protein of Arabidopsisthaliana P56780

Format: Lyophilized

Description: The PsbH protein was originally named 10- or 9-kDa phosphoprotein in higherplant chloroplasts. It is encoded by the plastome in algae and higher plants. PsbHis also present in cyanobacteria, where it exhibits 56% amino acid identity with thecorresponding protein from Arabidopsis. The protein contains 63–90 amino acids,depending on the species, with molecular masses between 7.0 and 9.9 kDa.PsbH is an intrinsic membrane protein with a single transmembrane helix and itsN-terminal region has been suggested to be exposed to the stromal side of thethylakoid membrane. Presence of PsbH already present in etiolated tissue canindicate that the protein may be involved in early stages of PSII assembly.Obtained biochemical data from PSII complexes isolated from spinach suggestthat PsbH, together with other PSII phosphoproteins, may be required for D1protein turnover by regulating dimeric and monomeric PSII transition through theirphosphorylation and dephosphorylation.

Specificity: Predicted reactivity: dicots including: Glycine max, Pisum sativum, monocots including: Oryza sativa,trees: Picea sitchensis, Populus deltoides.

Application Details

Application Notes: Recommended Dilution 1 : 5000 with standard ECL (WB).

Purity: Serum

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

From left to right: Arabidopsis thaliana, Horderum vulgare, Chlamydmononas reinhardtii, Synechococcus sp. 7942 (2 ug of total cellular protein was loaded per lane) Antibodies have also reactivity to thylakoids from: spinach, maize and conifers (data not presented).

Publications

Publications: Zghidi-Abouzid, Merendino, Buhr et al.: "Characterization of plastid psbT sense and antisense RNAs." in: Nucleic acids research, Vol. 39, Issue 13, pp. 5379-87, 2011 (PubMed).