DNAJC5 antibody (AA 182-198)

Catalog No. ABIN1742330

There are 5+ publications for this product available. The Rabbit Polyclonal anti-DNAJC5 antibody is suitable to detect DNAJC5 in samples from Rat, Mouse and Chicken. It has been validated for WB, IHC, IP and ICC.

Quick Overview for DNAJC5 antibody (AA 182-198) (ABIN1742330)

Target: DNAJC5 (DnaJ (Hsp40) Homolog, Subfamily C, Member 5 (DNAJC5))

Reactivity: Rat, Mouse, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNAJC5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Grade: KO Validated

Product Details anti-DNAJC5 Antibody

Binding Specificity: AA 182-198

Purpose: Rabbit anti-CSP antibody

Specificity: Specific for CSP.

Cross-Reactivity (Details): Reacts with: rat (P60905), mouse (P60904), chicken. Other species not tested yet.

Purification: affinity purified

Immunogen: Synthetic peptide corresponding to AA 182 to 198 from rat CSP (UniProt Id: P60905)

Application Details

Application Notes: WB:1 : 1000 up to 1 : 10000 , IP:yes ICC:1 : 100 up to 1 : 1000, IHC:1 : 500, IHC-P:not tested yet

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

References for anti-DNAJC5 antibody (ABIN1742330)

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Sharrad, Gai, Brookes et al.: "Selective coexpression of synaptic proteins, ?-synuclein, cysteine string protein-?, synaptophysin, synaptotagmin-1, and synaptobrevin-2 in vesicular acetylcholine transporter-immunoreactive axons in ..." in: The Journal of comparative neurology, Vol. 521, Issue 11, pp. 2523-37, (2013) (PubMed).

Lim, Kehm, Lee, Soper, Li, Trojanowski, Lee: "α-Syn suppression reverses synaptic and memory defects in a mouse model of dementia with Lewy bodies." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 27, pp. 10076-87, (2011) (PubMed).

García-Junco-Clemente, Cantero, Gómez-Sánchez, Linares-Clemente, Martínez-López, Luján, Fernández-Chacón: "Cysteine string protein-alpha prevents activity-dependent degeneration in GABAergic synapses." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 21, pp. 7377-91, (2010) (PubMed).

Wahlin, Moreira, Huang, Yu, Adler: "Molecular dynamics of photoreceptor synapse formation in the developing chick retina." in: The Journal of comparative neurology, Vol. 506, Issue 5, pp. 822-37, (2007) (PubMed).

Target Details for DNAJC5

Target: DNAJC5 (DnaJ (Hsp40) Homolog, Subfamily C, Member 5 (DNAJC5))

Alternative Name: CSP

Background:

Synonyms: Dnajc5, Cysteine string protein

Antigen Information: K.O. CSP is a chaperon at the synapse

UniProt: P60905

Pathways: Synaptic Vesicle Exocytosis