DNAJC5 antibody (C-Term)

Catalog No. ABIN1742330

The Rabbit Polyclonal anti-DNAJC5 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect DNAJC5 in samples from Human, Rat, Mouse, Cow, Chicken and Dog. There are 5+ publications available.

Quick Overview for DNAJC5 antibody (C-Term) (ABIN1742330)

Target: DNAJC5 (DnaJ (Hsp40) Homolog, Subfamily C, Member 5 (DNAJC5))

Reactivity: Human, Rat, Mouse, Cow, Chicken, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNAJC5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-DNAJC5 Antibody

Binding Specificity: AA 182-198, C-Term

Specificity: Specific for CSP.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Synthetic peptide of rat CSP (C-terminal part, aa 182-198) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.

Application Details

Application Notes: WB: 1 : 1000 up to 1 : 10000 (AP staining)
ICC: 1 : 100 up to 1 : 1000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References for anti-DNAJC5 antibody (ABIN1742330)

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Sharrad, Gai, Brookes et al.: "Selective coexpression of synaptic proteins, ?-synuclein, cysteine string protein-?, synaptophysin, synaptotagmin-1, and synaptobrevin-2 in vesicular acetylcholine transporter-immunoreactive axons in ..." in: The Journal of comparative neurology, Vol. 521, Issue 11, pp. 2523-37, (2013) (PubMed).

Lim, Kehm, Lee, Soper, Li, Trojanowski, Lee: "α-Syn suppression reverses synaptic and memory defects in a mouse model of dementia with Lewy bodies." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 27, pp. 10076-87, (2011) (PubMed).

García-Junco-Clemente, Cantero, Gómez-Sánchez, Linares-Clemente, Martínez-López, Luján, Fernández-Chacón: "Cysteine string protein-alpha prevents activity-dependent degeneration in GABAergic synapses." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 21, pp. 7377-91, (2010) (PubMed).

Wahlin, Moreira, Huang, Yu, Adler: "Molecular dynamics of photoreceptor synapse formation in the developing chick retina." in: The Journal of comparative neurology, Vol. 506, Issue 5, pp. 822-37, (2007) (PubMed).

Target Details for DNAJC5

Target: DNAJC5 (DnaJ (Hsp40) Homolog, Subfamily C, Member 5 (DNAJC5))

Alternative Name: CSP

Pathways: Synaptic Vesicle Exocytosis