SNAP91 antibody (AA 279-297)

Catalog No. ABIN1742332

This Rabbit Polyclonal antibody specifically detects SNAP91 in WB, IHC, IP and ICC. It exhibits reactivity toward Human, Rat, Mouse and Dog and has been mentioned in 4+ publications.

Quick Overview for SNAP91 antibody (AA 279-297) (ABIN1742332)

Target: SNAP91 (Synaptosomal-Associated Protein 91 (SNAP91))

Reactivity: Human, Rat, Mouse, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNAP91 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Grade: KO Validated

Product Details anti-SNAP91 Antibody

Binding Specificity: AA 279-297

Purpose: Rabbit anti-AP180 antibody

Specificity: Specific for AP 180.

Cross-Reactivity (Details): Reacts with: human (O60641), rat (Q05140), mouse (Q61548), dog. Other species not tested yet.

Purification: affinity purified

Immunogen: Synthetic peptide corresponding to AA 279 to 297 from rat AP180 (UniProt Id: Q05140)

Application Details

Application Notes: WB:1 : 1000 up to 1 : 5000 , IP:yes ICC:1 : 500, IHC:1 : 1000, IHC-P:not tested yet

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

References for anti-SNAP91 antibody (ABIN1742332)

Fuchs, Brandstätter, Regus-Leidig: "Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse." in: Frontiers in cellular neuroscience, Vol. 8, pp. 60, (2014) (PubMed).

Wilhelm, Mandad, Truckenbrodt, Kröhnert, Schäfer, Rammner, Koo, Claßen, Krauss, Haucke, Urlaub, Rizzoli: "Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins." in: Science (New York, N.Y.), Vol. 344, Issue 6187, pp. 1023-8, (2014) (PubMed).

Geis, Weishaupt, Hallermann, Grünewald, Wessig, Wultsch, Reif, Byts, Beck, Jablonka, Boettger, Üçeyler, Fouquet, Gerlach, Meinck, Sirén, Sigrist, Toyka, Heckmann, Sommer: "Stiff person syndrome-associated autoantibodies to amphiphysin mediate reduced GABAergic inhibition." in: Brain : a journal of neurology, Vol. 133, Issue 11, pp. 3166-80, (2010) (PubMed).

Zander, Münster-Wandowski, Brunk, Pahner, Gómez-Lira, Heinemann, Gutiérrez, Laube, Ahnert-Hilger: "Synaptic and vesicular coexistence of VGLUT and VGAT in selected excitatory and inhibitory synapses." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 22, pp. 7634-45, (2010) (PubMed).

Target Details for SNAP91

Target: SNAP91 (Synaptosomal-Associated Protein 91 (SNAP91))

Alternative Name: AP180

Background:

Synonyms: pp155, NP185, F1-20, SNAP 91, AP 3

Antigen Information: K.O. AP180 is a synaptic protein involved in clathrin mediated endocytosis

UniProt: Q05140