Chemokine (C-C Motif) Receptor 4 (CCR4) antibody

Details for Product No. ABIN179723
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Antigen
Synonyms A230103N10Rik, AA407540, AW456442, CCR4, RGD1310783, CC-CKR-4, CD194, CKR4, CMKBR4, ChemR13, HGCN:14099, K5-5, C-C CKR-4, CHEMR1, Cmkbr4, LESTR, Sdf1r
Reactivity
Human
(65), (31), (21), (5), (3), (1)
Host
Mouse (Murine)
(64), (9), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS), Cell-ELISA (cELISA)
(44), (39), (23), (15), (10), (8), (7), (6), (6), (2), (1)
Pubmed 5 references available
Quantity 100 µg
Shipping to United States (Change)
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Catalog No. ABIN179723
450.12 $
Plus shipping costs $45.00

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Immunogen immunisation with human CCR4 peptide
Clone KH-4F5
Isotype IgG2a
Specificity Anti-human CCR4
Purification Protein G
Alternative Name CCR4
Background CCR4 (CC chemokine receptor 4) molecule belongs to the rhodopsin family of G-protein-coupled receptors. Chemokine receptors are membrane bound molecules composed of 7-transmembrane domains and coupled to G-proteins. CCR4 binds the chemokines CCL17 (TARC) and CCL22 (MDC) and is highly expressed in most single-positive CD4(+) thymocytes especially in TH2- and regulatory T-cells. It plays a central role in T cell migration to the thymus, T cell maturation and education. CCR4 is often up-regulated in inflammation and cause conformational changes that trigger intracellular signaling pathways.
UniProt P51679
Research Area Cardiovascular, Atherosclerosis, Innate Immunity, Chemokines, Receptors
Application Notes Flow cytometry: 1.2 µg/10^6 cells
Immunofluorescence: 1 µg/10^6 cells
CELISA: 1:200 - 1:400
For each application a titration should be performed to determine the optimal concentration.
Comment

Synonyms: CC chemokine receptor 4

Restrictions For Research Use only
Concentration 2 mg/mL
Buffer PBS, pH 7.2
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C
Storage Comment short term: 2 °C - 8 °C, long term: -20 °C
Supplier Images
anti-Chemokine (C-C Motif) Receptor 4 (CCR4) antibody FACS analysis of BOSC23 cells using KH-4F5. BOSC23 cells were transientlytransfected with an expression vector encodingeither CCR4 (red curve) or an irrelevant protein(control transfectant). Binding of KH-4F5 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CCR4 transfected cells.
anti-Chemokine (C-C Motif) Receptor 4 (CCR4) antibody (2) Spectral Confocal Microscopy of CHO cells using KH-4F5. CHO cells were transiently transfected with an expression vector encoding CCR4. Binding of KH-4F5 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with A
anti-Chemokine (C-C Motif) Receptor 4 (CCR4) antibody (3) SDS-PAGE analysis of purified KH-4F5 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ug of purified KH-4F5 antibody. Proteins were separated by SDS-PAGE and stained with RAPID Stain Reagent.
General Imai, Baba, Nishimura et al.: "The T cell-directed CC chemokine TARC is a highly specific biological ligand for CC chemokine receptor 4." in: The Journal of biological chemistry, Vol. 272, Issue 23, pp. 15036-42, 1997 (PubMed).

Imai, Chantry, Raport et al.: "Macrophage-derived chemokine is a functional ligand for the CC chemokine receptor 4." in: The Journal of biological chemistry, Vol. 273, Issue 3, pp. 1764-8, 1998 (PubMed).

Murdoch, Finn: "Chemokine receptors and their role in inflammation and infectious diseases." in: Blood, Vol. 95, Issue 10, pp. 3032-43, 2000 (PubMed).

Andrew, Ruffing, Kim et al.: "C-C chemokine receptor 4 expression defines a major subset of circulating nonintestinal memory T cells of both Th1 and Th2 potential." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 1, pp. 103-11, 2001 (PubMed).

Purandare, Somerville: "Antagonists of CCR4 as immunomodulatory agents." in: Current topics in medicinal chemistry, Vol. 6, Issue 13, pp. 1335-44, 2006 (PubMed).

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