Carcinoembryonic Antigen-Related Cell Adhesion Molecule 19 (CEACAM19) antibody

Antigen: Carcinoembryonic Antigen-Related Cell Adhesion Molecule 19 (CEACAM19)

Synonyms: CEACAM19, MGC143231, CEAL1, MGC105097, MGC130130, MGC130155, MGC130156, C130022P09Rik

Clonality: Monoclonal (HY-8H10)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Cell-ELISA (cELISA)

Catalog no.: ABIN179727

Additional Information

Alternative name: CEACAM19

Immunogen: Genetic immunisation with cDNA encoding human CEACAM19

Format: Purified

Isotype: IgG1

Clone: HY-8H10

Description: CEA-related cell adhesion molecule 19 (CEACAM19) belongs to the carcinoembryonic antigen (CEA) gene family (1). It encodes a putative glycoprotein which is membrane-bound via a transmembrane domain. Like all members of the CEACAM family, the CEACAM19 protein is a single-pass type I membrane protein and consists of a single extracellular N domain. CEACAM19 expression is ubiquitous with highest expression in prostate, uterus, fetal brain, mammary gland, adrenal gland, skeletal muscle, small intestine, and kidney.

Application Details

Application Notes: FACS: 1.2ug/10^6 cells, Cell-ELISA: 1:200 to 1:400

Concentration: 2mg/ml

Purification: Protein G

Buffer: phosphate buffered saline, pH 7.2

Storage: Short term: 2°C - 8°C. long term: -20°C (avoid repeated freezing and thawing)

Restrictions: For Research Use only

Images

FACS analysis of BOSC23 cells using HY-8H10. BOSC23 cells were transiently trans-fected with an expression vector encoding either CEACAM19 (red curve) or an irrelevant protein (control transfectant). Binding of HY-8H10 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CEACAM19 transfected cells.

Spectral Confocal Microscopy of CHO cells usingHY-8H10. CHO cells were transientlytransfected with an expression vector encoding CEACAM19. Binding of HY-8H10 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled wi

SDS-PAGE analysis of purified HY-8H10 monoclonal antibody. Lane 1:molecular weight marker, Lane 2: 2 ug ofpurified HY-8H10 antibody. Proteins wereseparated by SDS-PAGE and stained withRAPID Stain Reagent.

Publications

Publications: Hammarstroem: "The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues." in: Seminars in cancer biology, Vol. 9, Issue 2, pp. 67-81, 1999 (PubMed).