Carcinoembryonic Antigen-Related Cell Adhesion Molecule 8 (CEACAM8) antibody

Antigen: Carcinoembryonic Antigen-Related Cell Adhesion Molecule 8 (CEACAM8)

Synonyms: CD67, CGM6, CD66b, NCA-95, CEACAM8, CEACAM1, MGC142545, cd67, cgm6, cd66b, nca-95

Clonality: Monoclonal (GM2H6)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Cell-ELISA (cELISA)

Catalog no.: ABIN179728

Additional Information

Alternative name: CEACAM8

Immunogen: Genetic immunization with cDNA encoding human CEACAM8

Format: Purified

Isotype: IgG1

Clone: GM2H6

Description: CEA-related cell adhesion molecule 8 (CEACAM8, CD66b) belongs to the carcinoembryonic antigen (CEA) gene family (1,3,4). It encodes a glycosylphosphatidylinositol (GPI)-linked glycoprotein with a Mr of 95,000 which is expressed in cells of the granulocyte-lineage. It is expressed in neutrophils and eosinophils and is characterized as a granulocyte-specific activation antigen. CEACAM8 could serve as a marker for granulocyte activities (2). Like all members of the CEA family, it consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by two immunoglobulin constant-like A and B do-mains.

Application Details

Application Notes: FACS: 1.2ug/10^6 cells, Cell-ELISA: 1:200 to 1:400

Purity: green curves). An irrelevant monoclonal antibody served asa negative control (black curves). For specificity testing, protein G-purified GM-2H6 was tested on all CEACAM transfectants. A positive signal was obtained only with CEACAM8 transfected cells (red curve).

Purification: PSG: BAP3

Storage: Short term: 2°C - 8°C. long term: -20°C (avoid repeated freezing and thawing)

Restrictions: For Research Use only

Images

FACS analysis of BOSC23 cells using GM2H6. BOSC23 cells were transiently transfected with an expression vector encoding either CEACAM8 (red curve) or an irrelevant protein (control transfectant). Binding of GM-0512 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CEACAM8 transfected cells.

BOSC23 cells were transiently transfected with expression vectorscontaining either the cDNA of CEACAM1, CEACAM3-8 or PSG. The latter expressed as a membrane bound fusion protein. Expression of the constructswas tested with monoclonal antibodies known to recognize the corresponding proteins (CEACAM1,3,4,5 and 6: D14HD11

Publications

Publications: Hammarstroem: "The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues." in: Seminars in cancer biology, Vol. 9, Issue 2, pp. 67-81, 1999 (PubMed).

Zhao, Xu, Fjaertoft et al.: "An enzyme-linked immunosorbent assay for human carcinoembryonic antigen-related cell adhesion molecule 8, a biological marker of granulocyte activities in vivo." in: Journal of immunological methods, Vol. 293, Issue 1-2, pp. 207-14, 2004 (PubMed).