SERPINB9 antibody

Catalog No. ABIN180930

The Mouse Monoclonal anti-SERPINB9 antibody (Clone 7D8) (ABIN180930) specifically detects SERPINB9 in WB, FACS and IHC (p). The antibody is reactive with Human samples.

Quick Overview for SERPINB9 antibody (ABIN180930)

Target: SERPINB9 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 9 (SERPINB9))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SERPINB9 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 7D8

Product Details anti-SERPINB9 Antibody

Specificity: This antibody is specific for SERPINB9/PI-9 (Proteinase Inhibitor 9). Does not react with Mouse and Pig.

Characteristics: Synonyms: PI9, Serpin B9, Cytoplasmic antiproteinase 3, CAP-3, CAP3, Proteinase inhibitor 9

Purification: Affinity Chromatography on Protein G

Immunogen: Recombinant PI-9 produced in P. pastoris. Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS-1myeloma cell line. Genename: PI-9

Isotype: IgG1

Application Details

Application Notes: Western Blot: This antibody detects a band of approx. 42 kda under Reducing ConditionsFlow Cytometry: Use 10 μL of 1/10-1/100 diluted antibody to label 1x10^6 cells in 100 μL. Membrane permeabilisation is required for this application (The use of Leucoperm isrecommended for this purpose). Immunohistochemistry on Paraffin Sections: 1/50. This product requires antigen retrieval using heat treatment prior to staining of paraffinsections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Tonsil. Also Suitable for use in Indirect Immunofluorescence. (See Ref.2 for more details)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for SERPINB9

Target: SERPINB9 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 9 (SERPINB9))

Alternative Name: SERPINB9

Background: PI-9 is a 42 kDa intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells. Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virusinfected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves.Synonyms: CAP-3, CAP3, Cytoplasmic antiproteinase 3, PI-9, PI9, Proteinase inhibitor 9, Serpin B9

Gene ID: 5272

UniProt: P50453