WISP2 antibody (Biotin)

Catalog No. ABIN181622

This Rabbit Polyclonal antibody specifically detects WISP2 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for WISP2 antibody (Biotin) (ABIN181622)

Target: WISP2 (WNT1 Inducible Signaling Pathway Protein 2 (WISP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WISP2 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-WISP2 Antibody (Biotin)

Specificity: This antibody detects Connective Tissue Growth Factor-Like protein.

Purification: Affinity chromatography

Immunogen: Highly pure (> 98 %) recombinant human CTGFL/WISP-2

Application Details

Application Notes: ELISA: Direct: To detect hCTGFL/WISP-2 by direct ELISA (using 100 μL/well antibodysolution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunctionwith compatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hCTGFL/WISP-2. Sandwich: To detect hCTGFL/WISP-2 by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunctionwith Polyclonal Anti-Human CTGFL/WISP-2 as a capture antibody, it allows the detection ofat least 0.2 - 0.4 ng/well of recombinant hCTGFL/WISP-2. Western Blot: To detect hCTGFL/WISP-2 by Western Blot analysis this antibody can be usedat a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hCTGFL/WISP-2 is 1.5 - 3.0 ng/lane, undereither reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for WISP2

Target: WISP2 (WNT1 Inducible Signaling Pathway Protein 2 (WISP2))

Alternative Name: WISP2

Background: Synonyms: CCN5, CT58, CTGF-L, CTGFL, Connective tissue growth factor-like protein, Connective tissue growth factor-related protein 58, UNQ228/PRO261, WISP-2, WNT1-inducible-signaling pathway protein 2

Gene ID: 8839

NCBI Accession: NP_003872

UniProt: O76076

Pathways: WNT Signaling, Growth Factor Binding