SPIB antibody (C-Term)

Catalog No. ABIN182361

The Rabbit Polyclonal anti-SPIB antibody (ABIN182361) specifically detects SPIB in WB. The antibody is reactive with Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio) samples.

Quick Overview for SPIB antibody (C-Term) (ABIN182361)

Target: SPIB (Spi-B Transcription Factor (Spi-1/PU.1 Related) (SPIB))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPIB antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SPIB Antibody

Binding Specificity: C-Term

Purpose: SPIB Antibody - C-terminal region

Sequence: GQQKGNRKRM TYQKLARALR NYAKTGEIRK VKRKLTYQFD SALLPAVRRA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against SPIB. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human SPIB

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP31422-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for SPIB

Target: SPIB (Spi-B Transcription Factor (Spi-1/PU.1 Related) (SPIB))

Alternative Name: SPIB

Background:

Background Information: SPI1 (MIM 165170) and SPIB are members of a subfamily of ETS (see ETS1, MIM 164720) transcription factors. ETS proteins share a conserved ETS domain that mediates specific DNA binding. SPIB and SPI1 bind to a purine-rich sequence, the PU box (5-prime-GAGGAA-3-prime).

Gene Name: Spi-B transcription factor (Spi-1/PU.1 related)

Alternative Symbols: SPI-B

Protein Name: Transcription factor Spi-B

Molecular Weight: 19kDa

Gene ID: 6689

NCBI Accession: NP_003112

UniProt: Q01892