Zinc Finger Protein 163 (ZNF163) antibody

Antigen: Zinc Finger Protein 163 (ZNF163)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Dog (Canine), Human, Rat (Rattus), Mouse (Murine)

Application: Western Blotting (WB)

Catalog no.: ABIN182605

Additional Information

Alternative name: ZNF163

Gene ID: GFI1

UniProt: Q99684

Immunogen: The immunogen for anti-GFI1 antibody: synthetic peptide directed towards the N terminal of human GFI1

Sequence: VPAPSRADSTSNAGGAKAEPRDRLSPESQLTEAPDRASAS PDSCEGSVCE

Cross-Reactivity: Dog (Canine), Human, Mouse (Murine), Rat (Rattus)

Format: Lyophilized

Reconstitution: Add 50 µl of distilled water. Final anti-GFI1 antibody concentration is 1 mg/ml.

Description: GFI1 may be a transcription factor involved in regulating the expression of genes active in the S phase during cell cycle progression in T-cells. GFI1 may be involved in tumor progression. Defects in GFI1 are a cause of autosomal dominant severe congenital neutropenia (SCN) and dominant nonimmune chronic idiopathic neutropenia of adults (NI-CINA) This gene encodes a nuclear zinc finger protein that functions as a transcriptional repressor. This protein plays a role in diverse developmental contexts, including hematopoiesis and oncogenesis. It functions as part of a complex along with other cofactors to control histone modifications that lead to silencing of the target gene promoters. Mutations in this gene cause autosomal dominant severe congenital neutropenia, and also dominant nonimmune chronic idiopathic neutropenia of adults, which are heterogeneous hematopoietic disorders that cause predispositions to leukemias and infections. Multiple alternatively spliced variants, encoding the same protein, have been identified for this gene.

Characteristics: Antigen Size: 422 amino acids

Molecular Weight: 45kDa

Application Details

Application Notes: WB Suggested Anti-GFI1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: Human Lung.

Purification: Affinity Purified

Buffer: PBS

Storage (Long Term): For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Storage (Short Term): Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.

Restrictions: For Research Use only

Images

WB Suggested Anti-GFI1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: Human Lung

Publications

Product: Doan, Porter, Duan et al.: "Targeted transcriptional repression of Gfi1 by GFI1 and GFI1B in lymphoid cells." in: Nucleic acids research, Vol. 32, Issue 8, pp. 2508-19, 2004 (PubMed).