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CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL) antibody

Antigen

CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL)

Synonyms CTCFL, Boris, RP23-44L6.1, OTTMUSG00000016680, BORIS, CT27, CTCF-T, MGC163358, dJ579F20.2, AW108038, CTCF, im:7152666, xctcf
Clonality Polyclonal
Host
Alternatives

Rabbit

Reactivity
Alternatives

Human

Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB)
1 reference available
Catalog no. ABIN183293
Quantity 50 µg  (Variants)
Price 317.90 $   Plus shipping costs $45.00
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Additional Information

Alternative name CTCFL / BORIS
Gene ID CTCFL
UniProt Q8NI51
Immunogen The immunogen for anti-CTCFL antibody: synthetic peptide directed towards the N terminal of human CTCFL
Sequence RSDEIVLTVSNSNVEEQEDQPTAGQADAEKAKSTKNQRKT KGAKGTFHCD
Cross-Reactivity Human
Format Lyophilized
Reconstitution Add 50 µl of distilled water. Final anti-CTCFL antibody concentration is 1 mg/ml.
Description CTCFL is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes. CTCFL is normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation.
Characteristics Antigen Size: 663 amino acids
Molecular Weight 76kDa

Application Details

Application Notes WB Suggested Anti-CTCFL Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Jurkat cell lysate.
Purification Affinity Purified
Buffer PBS
Storage (Long Term) For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
Storage (Short Term) Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.
Restrictions For Research Use only

Publications

Product Hong, Kang, Abdullaev et al.: "Reciprocal binding of CTCF and BORIS to the NY-ESO-1 promoter coincides with derepression of this cancer-testis gene in lung cancer cells." in: Cancer research, Vol. 65, Issue 17, pp. 7763-74, 2005 (PubMed).

Alternatives

Alternatives for antigen "CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL)", type "Antibodies"
Hosts Rabbit (33), Mouse (3), Rat (1)
Reactivities Human (35), Rat (Rattus) (25), Mouse (Murine) (24), Cow (Bovine) (20), Dog (Canine) (19), Pig (Porcine) (19), Horse (Equine) (18), Cat (Feline) (1), Chicken (1), Chimpanzee (1), Rabbit (1)
Applications Western Blotting (WB) (22), Immunofluorescence (IF) (21), ELISA (14), Immunohistochemistry (IHC) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
Epitopes AA 9-26 (2), N-Term (2)