GATA Zinc Finger Domain Containing 2B (GATAD2B) antibody

Antigen: GATA Zinc Finger Domain Containing 2B (GATAD2B)

Synonyms: P66beta, FLJ37346, KIAA1150, MGC138257, MGC138285, RP11-216N14.6, RGD1308533, GATAD2B

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Zebrafish

Application: Western Blotting (WB)

Catalog no.: ABIN183798

Additional Information

Alternative name: GATAD2B / Hp66 beta

Gene ID: GATAD2B

UniProt: Q8WXI9

Immunogen: The immunogen for anti-GATAD2B antibody: synthetic peptide directed towards the N terminal of human GATAD2B

Sequence: MDRMTEDALRLNLLKRSLDPADERDDVLAKRLKMEGHEAM ERLKMLALLK

Cross-Reactivity: Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)

Format: Lyophilized

Reconstitution: Add 50 µl of distilled water. Final anti-GATAD2B antibody concentration is 1 mg/ml.

Description: Hp66beta (GATAD2B), previously identified as a component of the human MeCP1 complex, is a second member of a p66 gene family (hp66alpha and hp66beta). Both proteins interact with MBD2 and MBD3 functionally and biochemically. Both factors function in synergy, since knocking-down p66alpha affects the repressive function of p66beta and vice versa. Furthermore, confocal microscopy shows a nuclear colocalization of hp66alpha with hp66beta and MBD2 in a speckled pattern. These two highly related mammalian p66 proteins display overlapping functions and are involved in methylation dependent transcriptional repression.

Characteristics: Antigen Size: 593 amino acids

Molecular Weight: 65kDa

Application Details

Application Notes: WB Suggested Anti-GATAD2B Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: HepG2 cell lysate.

Purification: Affinity Purified

Buffer: PBS

Storage (Long Term): For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Storage (Short Term): Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.

Restrictions: For Research Use only

Images

WB Suggested Anti-GATAD2B Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: HepG2 cell lysate

Publications

Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).