Signal Recognition Particle 14kDa (Homologous Alu RNA Binding Protein) (SRP14) (N-Term) antibody

Details for Product No. ABIN183958
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Antigen
Synonyms zgc:112290, 14kDa, AW536328, ALURBP
Epitope
N-Term
(7), (6), (2), (2), (1), (1), (1), (1)
Reactivity
Dog (Canine), Mouse (Murine), Rat (Rattus), Cow (Bovine), Xenopus laevis, Zebrafish (Danio rerio), Human, Chicken
(18), (3), (3), (1), (1), (1), (1)
Host
Rabbit
(19)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(19), (8), (6), (4)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN183958
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Immunogen Synthetic peptide directed towards the N terminal of human SRP14
Sequence KPIPKKGTVE GFEPADNKCL LRATDGKKKI STVVSSKEVN KFQMAYSNLL
Predicted Reactivity African clawed frog : 100 %, Bovine : 100 %, Chicken : 100 %, Dog : 100 %, Human : 100 %, Mouse : 90 %, Rat : 90 %, Zebrafish : 80 %
Characteristics This is a rabbit polyclonal antibody against SRP14. It was validated on Western Blot and immunohistochemistry.
Purification Protein A purified
Alternative Name SRP14
Background SRP14 belongs to the SRP14 family. The signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. The complex of SRP9 and SRP14 is required for SRP RNA binding.
Molecular Weight 15 kDa
Gene ID 6727
NCBI Accession NM_003134, NP_003125
UniProt P37108
Research Area Chromatin and Nuclear Signaling, Chromatin Binding Proteins
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 136 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Signal Recognition Particle 14kDa (Homologous Alu RNA Binding Protein) (SRP14) (N-Term) antibody WB Suggested Anti-SRP14 Antibody Titration: 1.25ug/ml
Positive Control: HepG2 cell lysate
anti-Signal Recognition Particle 14kDa (Homologous Alu RNA Binding Protein) (SRP14) (N-Term) antibody (2) anti-Signal Recognition Particle 14kDa (Homologous Alu RNA Binding Protein) (SRP14) (N-Term) antibody (Image 2)
Background publications Weichenrieder, Wild, Strub et al.: "Structure and assembly of the Alu domain of the mammalian signal recognition particle." in: Nature, Vol. 408, Issue 6809, pp. 167-73, 2000 (PubMed).

Validation Images
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