PRKRA antibody (Middle Region)

Catalog No. ABIN183963

Cited in 1 publication. The Rabbit Polyclonal anti-PRKRA antibody (ABIN183963) specifically detects PRKRA in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit and Horse samples.

Quick Overview for PRKRA antibody (Middle Region) (ABIN183963)

Target: PRKRA (Protein Kinase, Interferon-Inducible Double Stranded RNA Dependent Activator (PRKRA))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKRA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PRKRA Antibody

Binding Specificity: Middle Region

Sequence: RLPEYTLSQE GGPAHKREYT TICRLESFME TGKGASKKQA KRNAAEKFLA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against PRKRA. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PRKRA

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 313 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PRKRA antibody (ABIN183963)

Yoshida, Okamura, Hoshino, Shono, Yoshioka, Hinode, Yoshida: "Interaction between PKR and PACT mediated by LPS-inducible NF-?B in human gingival cells." in: Journal of cellular biochemistry, Vol. 113, Issue 1, pp. 165-73, (2011) (PubMed).

Target Details for PRKRA

Target: PRKRA (Protein Kinase, Interferon-Inducible Double Stranded RNA Dependent Activator (PRKRA))

Alternative Name: PRKRA

Background: PRKRA contains 3 DRBM (double-stranded RNA-binding) domains. It appears to have a pro-apoptotic function that may be suppressed in the presence of growth factor and activates EIF2AK2 in absence of double stranded RNA (dsRNA).
Alias Symbols: RAX, PACT, DYT16, HSD14
Protein Interaction Partner: ZMAT3, PRKRA, TARBP2, LYAR, STAU1, UBC, H1F0, EIF2AK2, CCDC124, C1orf35, DDX50, NOC3L, NOP16, STAU2, ZNF346, GTPBP4, DICER1, RBM34, EBNA1BP2, IFRD2, TCOF1, BMI1, SRPK3, POLR2G, MDC1, CAND1, UBE2I, SIRT7, ZFP28, KIAA1377, NKRF, SNX5, RABEPK, CEBPZ, SHC1, Z
Protein Size: 313

Molecular Weight: 34 kDa

Gene ID: 8575

NCBI Accession: NM_003690, NP_003681

UniProt: O75569

Pathways: Regulatory RNA Pathways