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Antibodies
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anti-Nanog Homeobox (NANOG) antibody
Nanog Homeobox (NANOG) antibody
| Antigen |
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Conjugate
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Biotin (6), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Application
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Western Blotting (WB) (87), ELISA (63), Immunofluorescence (IF) (35), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (17), Immunohistochemistry (IHC) (12), Immunocytochemistry (ICC) (7), Immunoprecipitation (IP) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (Dot) (2), Blocking Antibody (Inhibition) (1), Enzyme Immunoassay (EIA) (1), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN184267 |
| Quantity |
0.1 mg (Variants) |
| Price |
251.90 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Will be delivered in 2 to 3 Business Days |
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Alternative name
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NANOG
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Gene ID
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NANOG
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UniProt
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Q6JZS5
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Immunogen
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The immunogen for anti-NANOG antibody: synthetic peptide directed towards the N terminal of human NANOG
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Sequence
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PMPVICGPEENYPSLQMSSAEMPHTETVSPLPSSMDLLIQ DSPDSSTSPK
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Cross-Reactivity
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Human
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Format
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Lyophilized
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Reconstitution
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Add 100 µl of distilled water. Final anti-NANOG antibody concentration is 1 mg/ml.
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Description
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NANOG is a new marker for testicular carcinoma in situ and germ cell tumors. Gene knockdown of Nanog promotes differentiation, thereby demonstrating a role for these factors in human embryonic stem cell self-renewal.
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Characteristics
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Antigen Size: 305 amino acids
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Molecular Weight
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35kDa
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Purification
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Protein A purified
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Buffer
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PBS
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Storage (Long Term)
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For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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Storage (Short Term)
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Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.
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Restrictions
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For Research Use only
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Product
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Piestun, Kochupurakkal, Jacob-Hirsch et al.: "Nanog transforms NIH3T3 cells and targets cell-type restricted genes." in: Biochemical and biophysical research communications, Vol. 343, Issue 1, pp. 279-85, 2006 (PubMed).
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Alternatives for antigen "Nanog Homeobox (NANOG)", type "Antibodies"
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Hosts
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Rabbit (78), Mouse (21), Goat (9)
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Reactivities
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Human (102), Mouse (Murine) (31), Rat (Rattus) (22), Cow (Bovine) (2), Dog (Canine) (2), Pig (Porcine) (2), Cat (Feline) (1), Chicken (1), Goat (1), Monkey (1)
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Applications
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Western Blotting (WB) (87), ELISA (63), Immunofluorescence (IF) (35), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (17), Immunohistochemistry (IHC) (12), Immunocytochemistry (ICC) (7), Immunoprecipitation (IP) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (Dot) (2), Blocking Antibody (Inhibition) (1), Enzyme Immunoassay (EIA) (1), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Biotin (6), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Epitopes
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Internal Region (6), N-Term (6), C-Term (5), AA 1-154 (3), AA 1-50 (2), AA 20-166 (2), Ser285 (2), Center (1), Middle Region (1), pSer285 (1), pSer71 (1)
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