GCM1 antibody (C-Term)

Catalog No. ABIN184316

The Rabbit Polyclonal anti-GCM1 antibody has been validated for WB. It is suitable to detect GCM1 in samples from Human, Dog, Horse and Rabbit. There is 1 publication available.

Quick Overview for GCM1 antibody (C-Term) (ABIN184316)

Target: GCM1 (Glial Cells Missing Homolog 1 (GCM1))

Reactivity: Human, Dog, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCM1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GCM1 Antibody

Binding Specificity: C-Term

Sequence: DFNSYVQSPA YHSPQEDPFL FTYASHPHQQ YSLPSKSSKW DFEEEMTYLG

Cross-Reactivity: Dog (Canine), Human

Predicted Reactivity: Dog: 90%, Horse: 92%, Human: 100%, Rabbit: 81%

Characteristics: This is a rabbit polyclonal antibody against GCM1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human GCM1

Application Details

Application Notes: WB Suggested Anti-GCM1 Antibody Titration: 0.2-1 µg/mL
Positive Control: HepG2 cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 436 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-GCM1 antibody (ABIN184316)

Drewlo, Czikk, Baczyk, Lye, Kingdom: "Glial cell missing-1 mediates over-expression of tissue inhibitor of metalloproteinase-4 in severe pre-eclamptic placental villi." in: Human reproduction (Oxford, England), Vol. 26, Issue 5, pp. 1025-34, (2011) (PubMed).

Target Details for GCM1

Target: GCM1 (Glial Cells Missing Homolog 1 (GCM1))

Alternative Name: GCM1 / GCMA

Background: GCM1 is a DNA-binding protein with a gcm-motif (glial cell missing motif). GCM1 is a homolog of the Drosophila glial cells missing gene (gcm). This protein binds to the GCM-motif (A/G)CCCGCAT, a novel sequence among known targets of DNA-binding proteins.
Alias Symbols: GCMA, hGCMa
Protein Interaction Partner: FBXW2, UBC, SENP1, CAMK1, SUMO1, DUSP23, CREBBP, HDAC5, HDAC4, HDAC3, HDAC1, CUL1, SKP1, UBE2I,
Protein Size: 436

Molecular Weight: 49 kDa

Gene ID: 8521

NCBI Accession: NM_003643, NP_003634

UniProt: Q9NP62