GTF2E1 antibody (C-Term)

Catalog No. ABIN184330

This Rabbit Polyclonal antibody specifically detects GTF2E1 in WB and IHC. It exhibits reactivity toward Mouse, Rat, Cow, Dog, Horse, Guinea Pig and Rabbit.

Quick Overview for GTF2E1 antibody (C-Term) (ABIN184330)

Target: GTF2E1 (General Transcription Factor IIE, Polypeptide 1, alpha 56kDa (GTF2E1))

Reactivity: Mouse, Rat, Cow, Dog, Horse, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTF2E1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GTF2E1 Antibody

Binding Specificity: C-Term

Sequence: VADDPIVMVA GRPFSYSEVS QRPELVAQMT PEEKEAYIAM GQRMFEDLFE

Cross-Reactivity: Cow (Bovine), Dog (Canine), Fruit Fly (Drosophila melanogaster), Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against GTF2E1. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human GTF2E1

Application Details

Application Notes: WB Suggested Anti-GTF2 E1 Antibody Titration: 0.2-1 µg/mL
Positive Control: Daudi cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 439 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for GTF2E1

Target: GTF2E1 (General Transcription Factor IIE, Polypeptide 1, alpha 56kDa (GTF2E1))

Alternative Name: GTF2E1 / TF2E1

Background: The general transcription factor TFIIE has an essential role in eukaryotic transcription initiation together with RNA polymerase II and other general factors. Human TFIIE consists of two subunits of relative molecular mass 57,000 (TFIIE-alpha) and 34,000 (TFIIE-beta) and joins the preinitiation complex after RNA polymerase II and TFIIF. TFIIE-alpha is necessary for transcription initiation together with TFIIE-beta, and recombinant TFIIE-alpha can fully replace the natural subunit in an in vitro transcription assay. Its sequence contains several interesting structural motifs (leucine repeat, zinc finger and helix-turn-helix) and sequence similarities to bacterial sigma factors that suggest direct involvement in the regulation of transcription initiation
Alias Symbols: FE, TF2E1, TFIIE-A
Protein Interaction Partner: UBC, AARSD1, ACBD3, XPO5, SH3GLB2, OXR1, NSUN2, RPUSD2, SNF8, EFTUD2, TTC1, SH3GL1, SARS, HARS, SRPK2, TCEA1, GTF2H1, GTF2E2, CDK7, APP, tat, GTF2F1, ELAVL1, ZSCAN1, ZBTB9, TRIM15, PCBD2, TRIM29, CBFA2T2, IKBKG, ATF7IP, TAF6, GTF2B, TRIM24, SND1, GTF2H4,
Protein Size: 439

Molecular Weight: 49 kDa

Gene ID: 2960

NCBI Accession: NM_005513, NP_005504

UniProt: P29083