Sec61 alpha 1 Subunit (S. Cerevisiae) (SEC61A1) antibody

Antigen: Sec61 alpha 1 Subunit (S. Cerevisiae) (SEC61A1)

Synonyms: SEC61, HSEC61, SEC61A, Sec61a, AA408394, AA410007, sec61a1, MGC53497, MGC69436, SEC61A1, secY1, Sec61a1, ACYPI000055, DKFZp469H0814

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184559

Additional Information

Alternative name: SEC61A1

Gene ID: 29927

Swiss-Prot: NP_037468.1

Immunogen: Peptide with sequence C-KEQSEVGSMGALLF, from the C Terminus of the protein sequence according to NP_037468.1.

Cross-Reactivity: Human, Dog (Canine), Cow (Bovine), Pig (Porcine)

Format: Liquid

Grade: Serum-free

Description: Official Symbol: SEC61A1

Specificity: Human, Dog, Cow, Pig

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 48kDa band observed in Human Brain (Cerebelum) lysates (calculated MW of 52.3kDa according to NP_037468.1). Recommended concentration: 0.3-1µg/ml.

Concentration: 100µg/200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184559 staining (3µg/ml) of Daudi lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Goerlich, Prehn, Hartmann et al.: "A mammalian homolog of SEC61p and SECYp is associated with ribosomes and nascent polypeptides during translocation." in: Cell, Vol. 71, Issue 3, pp. 489-503, 1992 (PubMed).