Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1) (C-Term) antibody

Antigen: Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1)

Synonyms: ALDH1A1, ALDH1, MGC128391, AL1A1, aldc, aldh1, pumb1, aldh11, raldh1, aldh-e1, MGC107771, GGADHR, DKFZp469B0514, ALDDH, ALDC, PUMB1, ALDH11, RALDH1, ALDH-E1, MGC2318, Ahd2, Aldh1, Aldh2, E1, Ahd-2, Raldh1, Aldh1a2

Epitope: C-Term

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184581

Additional Information

Alternative name: ALDH1A1 (C Terminus)

Gene ID: 216

Immunogen: C-EVKTVTVKISQKNS

Format: Affinity-purified

Description: All names and symbols: ALDH1A1, aldehyde dehydrogenase 1 family, member A1, ALDC, ALDH-E1, ALDH1, ALDH11, MGC2318,, PUMB1, RALDH1, ALDH class 1, acetaldehyde dehydrogenase 1, aldehyde dehydrogenase 1, soluble, aldehyde dehydrogenase 1A1, aldehyde dehydrogenase, liver cytosolic, retinal dehydrogenase 1. Official Symbol: ALDH1A1. RefSeq number(s): NP_000680.2. Gene ontology terms: aldehyde dehydrogenase (NAD+) activity, androgen binding, electron transporter activity, aldehyde metabolism, cytosol, oxidoreductase activity

Specificity: This antibody may cross-react with ALDH1A2 (GeneID 8854) with one residue difference from this design and with ALDH2 (GeneID 217) with two residues difference from this design. Human, Mouse, Rat, Dog

Synonyms: ALDH1A1, ALDH1, MGC128391, AL1A1, aldc, aldh1, pumb1, aldh11, raldh1, aldh-e1, MGC107771, GGADHR, DKFZp469B0514, ALDDH, ALDC, PUMB1, ALDH11, RALDH1, ALDH-E1, MGC2318, Ahd2, Aldh1, Aldh2, E1, Ahd-2, Raldh1, Aldh1a2

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 55kDa band observed in human liver lysates (calculated MW of 54.9kDa according to NP_000680.2). Recommended concentration: 0.03-0.1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184581 staining (0.03µg/ml) of human liver lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

HEK293 overexpressing ALDH1A1 (RC200723) and probed with ABIN184581 (mock transfection in first lane), tested by Origene.

Publications

Publications: Zheng, Wang, Weiner: "Cloning and expression of the full-length cDNAS encoding human liver class 1 and class 2 aldehyde dehydrogenase." in: Alcoholism, clinical and experimental research, Vol. 17, Issue 4, pp. 828-31, 1993 (PubMed).