Methionine Synthase (MTR) antibody

Antigen: Methionine Synthase (MTR)

Synonyms: MS, cblG, FLJ33168, FLJ43216, FLJ45386, methioninesynthase, AI894170, D830038K18Rik

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184584

Additional Information

Alternative name: MTR

Gene ID: 4548, 81522 (rat)

Immunogen: C-VEKWLGPILGYDTD

Format: Affinity-purified

Description: All names and symbols: MTR, 5-methyltetrahydrofolate-homocysteine methyltransferase, 5-methyltetrahydrofolate-homocysteine methyltransferase 1. Official Symbol: MTR. RefSeq number(s): NP_000245. Gene ontology terms: homocysteine S-methyltransferase activity, methyltransferase activity, central nervous system development, intracellular, methionine biosynthesis, folic acid and derivative biosynthesis, transferase activity, 5-methyltetrahydrofolate-homocysteine S-methyltransferase activity, dihydropteroate synthase activity

Specificity: Human, Rat

Application Details

Application Notes: Western blot: Approx 150kDa band seen in HeLa and Jurkat cell lysates. Recommended for use at 1-3 µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors

Restrictions: For Research Use only

Images

ABIN184584 (2µg/ml) staining of Hela lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence.

Publications

Publications: Chen, Liu, Hwang et al.: "Human methionine synthase. cDNA cloning, gene localization, and expression." in: The Journal of biological chemistry, Vol. 272, Issue 6, pp. 3628-34, 1997 (PubMed).