MAX antibody (C-Term)

Catalog No. ABIN184586

The Goat Polyclonal anti-MAX antibody has been validated for WB, ELISA, IHC and IF. It is suitable to detect MAX in samples from Human.

Quick Overview for MAX antibody (C-Term) (ABIN184586)

Target: MAX (MYC Associated Factor X (MAX))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MAX antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Verified

Product Details anti-MAX Antibody

Binding Specificity: C-Term

Purpose: MAX

Sequence: EEPQSRKKLR MEAS

Specificity: This antibody is expected to recognize both reported isoforms (NP_002373.3, NP_660087.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EEPQSRKKLRMEAS, from the C Terminus of the protein sequence according to NP_002373.3, NP_660087.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 10 μg/mL.

Western Blot: Approx 22 kDa band observed in lysates of cell line Jurkat (calculated MW of 18.3 kDa according to NP_002373.3). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Expression of the protein seen in the cytoplasm and nuclei of A431 and U251 cells. Recommended concentration: 10μg/ml.

Additional validation: This antibody has been successfully used in the followin

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MAX

Target: MAX (MYC Associated Factor X (MAX))

Alternative Name: MAX

Background: MAX, MAX protein, MGC10775, MGC11225, MGC18164, MGC34679, MGC36767, myc-associated factor X, helix-loop-helix zipper protein, MYC associated factor X, bHLHd4, bHLHd5, bHLHd6, bHLHd7, bHLHd8, orf1

Gene ID: 4149, 17187

NCBI Accession: NP_002373, NP_660087

Pathways: Mitotic G1-G1/S Phases