Signal Transducing Adaptor Family Member 2 (STAP2) antibody

Antigen: Signal Transducing Adaptor Family Member 2 (STAP2)

Synonyms: BKS, FLJ20234, MGC108972, MGC127502

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184610

Additional Information

Alternative name: STAP2 / BKS

Gene ID: 55620

Immunogen: C-ELQKKLEKRRALEH

Format: Affinity-purified

Description: All names and symbols: STAP2, BKS, STAP-2, FLJ20234, breast tumor kinase substrate, brk kinase substrate, signal-transducing adaptor protein-2. Official Symbol: STAP2. RefSeq number(s): NP_060190.2, NP_001013863.1

Specificity: This antibody is expected to recognise both reported isoforms (as represented by NP_060190.2 and NP_001013863.1). Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 48kDa band observed in human heart lysates (calculated MW of 49.6kDa according to NP_060190.2). Recommended concentration: 0.05-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184610 (0.03µg/ml) staining of human heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Minoguchi, Minoguchi, Aki et al.: "STAP-2/BKS, an adaptor/docking protein, modulates STAT3 activation in acute-phase response through its YXXQ motif." in: The Journal of biological chemistry, Vol. 278, Issue 13, pp. 11182-9, 2003 (PubMed).