Dedicator of Cytokinesis 1 (DOCK1) antibody

Antigen: Dedicator of Cytokinesis 1 (DOCK1)

Synonyms: ced5, DOCK180, AI854900, 9130006G06Rik, D630004B07Rik, DOCK1, sph238, Zizimin2, 8030476J24, 5033414A21Rik, ACG, ZIZ2, FLJ32122, FLJ43653, bB128O4.1, MGC140073

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184630

Additional Information

Alternative name: DOCK1

Gene ID: 1793, 330662 (mouse)

Immunogen: C-TTRKQTSVDSGIVQ

Format: Affinity-purified

Description: All names and symbols: DOCK1, DOCK180, dedicator of cyto-kinesis 1. Official Symbol: DOCK1. RefSeq number(s): NP_001371. Gene ontology terms: GTPase activator activity, small GTPase mediated signal transduction, integrin-mediated signaling pathway, phagocytosis, engulfment, apoptosis, signal transduction, membrane, cytoskeleton

Specificity: Human, Mouse

Application Details

Application Notes: Western blot: Approx 150-200kDa band seen in A431 cell lysate. Recommended for use at 3-4µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling, Apoptosis/Necrosis

Restrictions: For Research Use only

Images

ABIN184630 staining (4µg/ml) of A431 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Hasegawa, Kiyokawa, Tanaka et al.: "DOCK180, a major CRK-binding protein, alters cell morphology upon translocation to the cell membrane." in: Molecular and cellular biology, Vol. 16, Issue 4, pp. 1770-6, 1996 (PubMed).