Nuclear Factor, Interleukin 3 Regulated (NFIL3) antibody

Antigen: Nuclear Factor, Interleukin 3 Regulated (NFIL3)

Synonyms: E4BP4, IL3BP1, NFIL3A, NF-IL3A, zgc:100867, NFIL3, MGC151767, e4bp4, il3bp1, nfil3a, nf-il3a, MGC107826

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184644

Additional Information

Alternative name: NFIL3

Gene ID: 4783, 18030 (mouse), 114519 (rat)

Immunogen: C-RLIATQPISASDSG

Format: Affinity-purified

Description: All names and symbols: NFIL3, nuclear factor, interleukin 3 regulated, E4BP4, IL3BP1, NFIL3A, NF-IL3A, interleukin-3 promoter transcriptional activator. Official Symbol: NFIL3. RefSeq number(s): NP_005375.2. Gene ontology terms: transcription co-repressor activity, transcription factor activity, regulation of transcription, DNA-dependent, immune response, transcription from Pol II promoter, nucleus

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx. 55-60kDa band observed in lysates of Jurkat and HepG2 (calculated MW of 51.5kDa according to NP_005375.2). The observed molecular weight corresponds to earlier findings in literature (Ikushima et al., Proc Natl Acad Sci U S A. 1997 Mar 18,94(6):2609-14., PMID: 9122243). Recommended concentration: 0.5-1.5µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors, Innate Immunity, Cytokines

Restrictions: For Research Use only

Images

ABIN184644 staining (0.5µg/ml) of HepG2 lysate (RIPA buffer, 20µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Zhang, Zhang, Kornuc et al.: "Molecular cloning and characterization of NF-IL3A, a transcriptional activator of the human interleukin-3 promoter." in: Molecular and cellular biology, Vol. 15, Issue 11, pp. 6055-63, 1995 (PubMed).