NFIL3 antibody (Middle Region)

Catalog No. ABIN2777219

The Rabbit Polyclonal anti-NFIL3 antibody (ABIN2777219) specifically detects NFIL3 in WB. The antibody is reactive with Human, Mouse, Rat, Cow, Sheep, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio) and Rabbit samples.

Quick Overview for NFIL3 antibody (Middle Region) (ABIN2777219)

Target: NFIL3 (Nuclear Factor, Interleukin 3 Regulated (NFIL3))

Reactivity: Human, Mouse, Rat, Cow, Sheep, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio), Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFIL3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NFIL3 Antibody

Binding Specificity: Middle Region

Purpose: NFIL3 Antibody - middle region

Sequence: GYSHSPPLLQ VNRSSSNSPR TSETDDGVVG KSSDGEDEQQ VPKGPIHSPV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Sheep: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against NFIL3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NFIL3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP31177-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for NFIL3

Target: NFIL3 (Nuclear Factor, Interleukin 3 Regulated (NFIL3))

Alternative Name: NFIL3

Background:

Background Information: NFIL3 is a basic leucine zipper (bZIP) transcription factor that represses or activates transcription in non-osteoblastic cells. NFIL3 play a role in attenuation of PTH target gene transcription in osteoblasts. NFIL3 repression domain interacts specifically with the TBP binding repressor protein Dr1.Glucocorticoid-mediated upregulation of the bZIP transcriptional repressor gene, NFIL3, is dependent on [Ca2+]i levels, and correlates with Glucocorticoid-evoked apoptosis of Glucocorticoid-sensitive CEM-C7-14 cells. NFIL3 is involved in a distinct growth factor-regulated signaling pathway that is responsible for the survival of early B-cell progenitors, and whose alteration by E2A-HLF leads to childhood B lineage leukemia.

Gene Name: Nuclear factor, interleukin 3 regulated

Alternative Symbols: E4BP4, IL3BP1, NFIL3A, NF-IL3A

Protein Name: Nuclear factor interleukin-3-regulated protein

Molecular Weight: 51kDa

Gene ID: 4783

NCBI Accession: NP_005375

UniProt: Q16649