Protein Phosphatase 2, Regulatory Subunit B', delta (PPP2R5D) antibody

Antigen: Protein Phosphatase 2, Regulatory Subunit B', delta (PPP2R5D)

Synonyms: B56D, MGC2134, MGC8949, Tex271, B'delta, TEG-271, zgc:73160, zgc:77529, PPP2R5D

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184648

Additional Information

Alternative name: PPP2R5D

Gene ID: 5528

Immunogen: C-KRAEEFLTASQEAL

Description: All names and symbols: PPP2R5D, MGC2134, MGC8949, protein phosphatase 2, regulatory subunit B (B56), delta isoform, PP2A, B subunit, B' delta isoform, PP2A, B subunit, R5 delta isoform, PP2A, B subunit, B56 delta isoform, PP2A, B subunit, PR61 delta isoform, Serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, delta isoform. Official Symbol: PPP2R5D. RefSeq number(s): NP_006236. Gene ontology terms: protein phosphatase type 2A, intrinsic regulator activity, neurogenesis, signal transduction, protein phosphatase type 2A complex, nucleus

Specificity: Human, Rabbit

Application Details

Application Notes: Western blot: Approx 70kDa and 60kDa bands seen in 293 lysate (Predicted MW of 68kDa and 62kDa according to NP_006236 and AAH01175, respectively). Recommended for use at 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Images

ABIN184648 staining (2µg/ml) of 293 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

McCright, Virshup: "Identification of a new family of protein phosphatase 2A regulatory subunits." in: The Journal of biological chemistry, Vol. 270, Issue 44, pp. 26123-8, 1995 (PubMed).