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Details for Product No. ABIN184664

CUB Domain Containing Protein 1 (CDCP1) (C-Term) antibody

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Antigen
Synonyms CD318, TRASK, SIMA135, 9030022E12Rik, AA409659, E030027H19Rik, RGD1305578
Epitope
»Alternatives C-Term
Reactivity
»Alternatives Human
Host
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Methode Type Peptide ELISA
Application
»Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 1 reference available
Catalog no. ABIN184664
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence C-PLLNTQEPMEPAE, from the C Terminus of the protein sequence according to NP_073753.3.
Specificity This antibody is expected to recognize isoform 1 (NP_073753.3) only.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CDCP1
Background Synonyms: CDCP1, CUB domain containing protein 1, HGNC:24357, CD318, SIMA135, TRASK, CUB domain-containing protein 1, transmembrane and associated with src kinases
Gene ID 64866
NCBI Accession NP_073753.3
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 90 kDa band observed in Human Colon lysates (calculated MW of 92.3 kDa according to NP_073753.3). Recommended concentration: 1-3 µg/mL. An additional band of unknown identity was also consistently observed at 200 kDa. This band was successfully blocked by incubation with the immunising peptide.
IHC: This antibody has been successfully used in IHC for human endometrium. Immunoprecipitation: This antibody has been successfully used in IP with A549 cells.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Hooper, Zijlstra, Aimes et al.: "Subtractive immunization using highly metastatic human tumor cells identifies SIMA135/CDCP1, a 135 kDa cell surface phosphorylated glycoprotein antigen." in: Oncogene, Vol. 22, Issue 12, pp. 1783-94, 2003 (PubMed).

Alternatives for antigen "CUB Domain Containing Protein 1 (CDCP1)", type "Antibodies"
Hosts (48), (24), (9), (1), (1)
Reactivities (80), (35), (17), (17), (1), (1)
Applications (49), (29), (24), (15), (13), (10), (5), (2), (1), (1)
Conjugates (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (17), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
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