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CUB Domain Containing Protein 1 (CDCP1) (C-Term) antibody

Details for Product No. ABIN184664, Supplier: Login to see
Antigen
  • CD318
  • SIMA135
  • TRASK
  • 9030022E12Rik
  • AA409659
  • E030027H19Rik
  • RGD1305578
Epitope
C-Term
41
15
10
6
5
5
4
4
4
3
2
2
1
1
1
1
1
1
1
Reactivity
Human
137
27
12
4
3
3
3
2
1
1
Host
Goat
78
40
21
2
2
Clonality
Polyclonal
Conjugate
Un-conjugated
8
6
5
3
3
3
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
92
49
25
20
17
16
6
3
2
2
2
1
1
Supplier
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Immunogen Peptide with sequence C-PLLNTQEPMEPAE, from the C Terminus of the protein sequence according to NP_073753.3.
Blocking Peptide Blocking peptide for this product available: ABIN369557
Specificity This antibody is expected to recognize isoform 1 (NP_073753.3) only.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CDCP1 (CDCP1 Antibody Abstract)
Background Synonyms: CDCP1, CUB domain containing protein 1, HGNC:24357, CD318, SIMA135, TRASK, CUB domain-containing protein 1, transmembrane and associated with src kinases
Gene ID 64866
NCBI Accession NP_073753
Pathways
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 90 kDa band observed in Human Colon lysates (calculated MW of 92.3 kDa according to NP_073753.3). Recommended concentration: 1-3 µg/mL. An additional band of unknown identity was also consistently observed at 200 kDa. This band was successfully blocked by incubation with the immunising peptide.
IHC: This antibody has been successfully used in IHC for human endometrium. Immunoprecipitation: This antibody has been successfully used in IP with A549 cells.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-CUB Domain Containing Protein 1 (CDCP1) (C-Term) antibody (ABIN184664) ABIN184664 (1µg/ml) staining of Human Colon lysate (35µg protein in RIPA buffer). Pri...
Background publications Hooper, Zijlstra, Aimes et al.: "Subtractive immunization using highly metastatic human tumor cells identifies SIMA135/CDCP1, a 135 kDa cell surface phosphorylated glycoprotein antigen." in: Oncogene, Vol. 22, Issue 12, pp. 1783-94, 2003 (PubMed).