CUB Domain Containing Protein 1 (CDCP1) (C-Term) antibody

Antigen: CUB Domain Containing Protein 1 (CDCP1)

Synonyms: CD318, TRASK, SIMA135

Epitope: C-Term

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184664

Additional Information

Alternative name: CDCP1 (isoform 1: C term)

Gene ID: 64866

Immunogen: C-PLLNTQEPMEPAE

Format: Affinity-purified

Description: All names and symbols: CDCP1, CUB domain containing protein 1 , HGNC:24357, CD318, SIMA135, TRASK , CUB domain-containing protein 1, transmembrane and associated with src kinases. Official Symbol: CDCP1. RefSeq number(s): NP_073753.3

Specificity: This antibody is expected to recognize isoform 1 (NP_073753.3) only. Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 90kDa band observed in Human Colon lysates (calculated MW of 92.3kDa according to NP_073753.3). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 200kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184664 (1µg/ml) staining of Human Colon lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing CDCP1 (RC220633) and probed with ABIN184664 (mock transfection in first lane), tested by Origene.

Publications

Publications: Hooper, Zijlstra, Aimes et al.: "Subtractive immunization using highly metastatic human tumor cells identifies SIMA135/CDCP1, a 135 kDa cell surface phosphorylated glycoprotein antigen." in: Oncogene, Vol. 22, Issue 12, pp. 1783-94, 2003 (PubMed).