CUB Domain Containing Protein 1 (CDCP1) (C-Term) antibody

Details for Product No. ABIN184664
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Antigen
Synonyms CD318, SIMA135, TRASK, 9030022E12Rik, AA409659, E030027H19Rik, RGD1305578
Epitope
C-Term
(19), (7), (6), (6), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(90), (34), (13), (12)
Host
Goat
(61), (22), (9), (1), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(64), (36), (18), (14), (12), (10), (4), (3), (2), (2), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN184664
374.27 $
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Immunogen Peptide with sequence C-PLLNTQEPMEPAE, from the C Terminus of the protein sequence according to NP_073753.3.
Specificity This antibody is expected to recognize isoform 1 (NP_073753.3) only.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CDCP1
Background Synonyms: CDCP1, CUB domain containing protein 1, HGNC:24357, CD318, SIMA135, TRASK, CUB domain-containing protein 1, transmembrane and associated with src kinases
Gene ID 64866
NCBI Accession NP_073753
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 90 kDa band observed in Human Colon lysates (calculated MW of 92.3 kDa according to NP_073753.3). Recommended concentration: 1-3 µg/mL. An additional band of unknown identity was also consistently observed at 200 kDa. This band was successfully blocked by incubation with the immunising peptide.
IHC: This antibody has been successfully used in IHC for human endometrium. Immunoprecipitation: This antibody has been successfully used in IP with A549 cells.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-CUB Domain Containing Protein 1 (CDCP1) (C-Term) antibody ABIN184664 (1µg/ml) staining of Human Colon lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Hooper, Zijlstra, Aimes et al.: "Subtractive immunization using highly metastatic human tumor cells identifies SIMA135/CDCP1, a 135 kDa cell surface phosphorylated glycoprotein antigen." in: Oncogene, Vol. 22, Issue 12, pp. 1783-94, 2003 (PubMed).

Validation Images
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