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CUB Domain Containing Protein 1 (CDCP1) (C-Term) antibody

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Synonyms CD318, SIMA135, TRASK, 9030022E12Rik, AA409659, E030027H19Rik, RGD1305578
(41), (15), (10), (6), (5), (5), (4), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
(136), (27), (12), (4), (3), (3), (3), (2), (1), (1)
(77), (40), (21), (2), (2)
(8), (6), (5), (3), (3), (3), (1), (1)
Methode Type Peptide ELISA
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(92), (49), (25), (20), (17), (16), (6), (3), (2), (2), (2), (1), (1)
Pubmed 1 reference available
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Quantity 100 μg
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Availability Available
Immunogen Peptide with sequence C-PLLNTQEPMEPAE, from the C Terminus of the protein sequence according to NP_073753.3.
Blocking Peptide Blocking peptide for this product available: ABIN369557
Specificity This antibody is expected to recognize isoform 1 (NP_073753.3) only.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name CDCP1 (CDCP1 Antibody Abstract)
Background Synonyms: CDCP1, CUB domain containing protein 1, HGNC:24357, CD318, SIMA135, TRASK, CUB domain-containing protein 1, transmembrane and associated with src kinases
Gene ID 64866
NCBI Accession NP_073753
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 90 kDa band observed in Human Colon lysates (calculated MW of 92.3 kDa according to NP_073753.3). Recommended concentration: 1-3 µg/mL. An additional band of unknown identity was also consistently observed at 200 kDa. This band was successfully blocked by incubation with the immunising peptide.
IHC: This antibody has been successfully used in IHC for human endometrium. Immunoprecipitation: This antibody has been successfully used in IP with A549 cells.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-CUB Domain Containing Protein 1 (CDCP1) (C-Term) antibody (ABIN184664) ABIN184664 (1µg/ml) staining of Human Colon lysate (35µg protein in RIPA buffer). Pri...
Background publications Hooper, Zijlstra, Aimes et al.: "Subtractive immunization using highly metastatic human tumor cells identifies SIMA135/CDCP1, a 135 kDa cell surface phosphorylated glycoprotein antigen." in: Oncogene, Vol. 22, Issue 12, pp. 1783-94, 2003 (PubMed).

Catalog No. ABIN184664
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