Dimethylarginine Dimethylaminohydrolase 1 (DDAH1) antibody

Antigen: Dimethylarginine Dimethylaminohydrolase 1 (DDAH1)

Synonyms: DDAH, FLJ21264, FLJ25539, AI987801, AW050362, 2410006N07Rik, 2510015N06Rik, fc30c11, wu:fc30c11, ddah1, MGC69055, DDAH1, MGC165853

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184669

Additional Information

Alternative name: DDAH1

Gene ID: 23576

Immunogen: TCCSVLINKKVDS

Format: Affinity-purified

Description: All names and symbols: DDAH1, dimethylarginine dimethylaminohydrolase 1, DDAH, DDAHI, NG, NG-dimethylarginine dimethylaminohydrolase, RP4-621F18.1, FLJ21264, FLJ25539. Official Symbol: DDAH1. RefSeq number(s): NP_036269.1. Gene ontology terms: enzyme, arginine catabolism, NO mediated signal transduction

Specificity: Human 285 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 37kDa band observed in human kidney, liver and pancreas lysates (calculated MW of 31.1kDa according to NP_036269.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pullamsetti et al, FASEB J. 2005 Jul,19(9):1175-7, PMID: 15827267). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Neurology

Restrictions: For Research Use only

Images

ABIN184669 staining (0.1µg/ml) of human kidney lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Kimoto, Miyatake, Sasagawa et al.: "Purification, cDNA cloning and expression of human NG,NG-dimethylarginine dimethylaminohydrolase." in: European journal of biochemistry / FEBS, Vol. 258, Issue 2, pp. 863-8, 1999 (PubMed).