PTGS2 antibody (C-Term)

Catalog No. ABIN184672

This Goat Polyclonal antibody specifically detects PTGS2 in WB, ELISA, IF and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for PTGS2 antibody (C-Term) (ABIN184672)

Target: PTGS2 (Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This PTGS2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-PTGS2 Antibody

Binding Specificity: C-Term

Purpose: COX2 / PTGS2

Sequence: NPTVLLKERS TEL

Cross-Reactivity: Cow, Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NPTVLLKERSTEL, from the C Terminus of the protein sequence according to NP_000954.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 75-80 kDa band observed in lysates of cell lines A549 and Daudi (predicted MW of 69 kDa according to NP_000954.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3 μg/mL. Primary incubatio

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and vesicles of HepG2 and NIH3T3 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommende

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PTGS2

Target: PTGS2 (Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2))

Alternative Name: PTGS2

Background: PTGS2, COX2, prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase), COX-2, PHS-2, PGG/HS, PGHS-2, hCox-2, prostaglandin G/H synthase and cyclooxygenase, GRIPGHS, cyclooxygenase 2b

Gene ID: 5743

NCBI Accession: NP_000954

Pathways: Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation