Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL) antibody

Antigen: Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL)

Synonyms: cbl, Cbl-2, c-Cbl, 4732447J05Rik, CBL2, C-CBL, RNF55

Clonality: Polyclonal

Host: Goat

Reactivity: Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184673

Additional Information

Alternative name: CBL

Gene ID: 867, 12402 (mouse)

Immunogen: C-REFVSISSPAHVAT

Format: Affinity-purified

Description: All names and symbols: CBL, CBL2, C-CBL, RNF55, Cas-Br-M (murine) ecotropic retroviral transforming sequence, Cas-Br-M ecotropic retroviral transforming sequence (Oncogene CBL2). Official Symbol: CBL. RefSeq number(s): NP_005179.2. Gene ontology terms: signal transducer activity, transcription factor activity, cell growth and/or maintenance, cell surface receptor linked signal transduction, nucleus, ligase activity

Specificity: Human, Mouse, Rat 906 aa

Application Details

Application Notes: Western blot: Approx 120kDa band seen in 3T3 lysate. Recommended for use at 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cancer, Transcription Factors, Proteolysis / Ubiquitin

Restrictions: For Research Use only

Images

ABIN184673 staining (2µg/ml) of 3T3 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Langdon, Hartley, Klinken et al.: "v-cbl, an oncogene from a dual-recombinant murine retrovirus that induces early B-lineage lymphomas." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 4, pp. 1168-72, 1989 (PubMed).