Engulfment and Cell Motility 1 (ELMO1) antibody

Antigen: Engulfment and Cell Motility 1 (ELMO1)

Synonyms: CED12, CED-12, ELMO-1, KIAA0281, MGC126406, ELMO1, MGC68475, zgc:85612, im:7151147, wu:fq24e08, MGC139838, DKFZp459C0528

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184680

Additional Information

Alternative name: ELMO1

Gene ID: 9844, 140580 (mouse)

Immunogen: PKEPSNYDFVYDCN

Format: Affinity-purified

Description: All names and symbols: ELMO1, engulfment and cell motility 1 (ced-12 homolog, C. elegans), CED12, KIAA0281, ced-12 homolog 1, engulfment and cell motility 1. Official Symbol: ELMO1. RefSeq number(s): NP_055615.8, NP_569709.1, NP_001034548.1

Specificity: This antibody is expected to recognise both human isoforms of this protein. NP_569709.1 and NP_001034548.1 represent the same isoform. Human, Mouse 247aa

Application Details

Application Notes: Western blot: Approx 70kDa and 50kDa bands seen in Jurkat cell lysate. Recommended for use at 1-2µg/ml. Note the smaller isoform of this protein has a consensus glycosylation site, which may explain the higher than expected band size (50 kDa versus 30 kDa). Glycosylation can affect migration of proteins on SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press].

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cancer, Apoptosis/Necrosis, Extracellular Matrix

Restrictions: For Research Use only

Images

ABIN184680 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Gumienny, Brugnera, Tosello-Trampont et al.: "CED-12/ELMO, a novel member of the CrkII/Dock180/Rac pathway, is required for phagocytosis and cell migration." in: Cell, Vol. 107, Issue 1, pp. 27-41, 2001 (PubMed).