Dual Specificity Phosphatase 10 (DUSP10) antibody

Antigen: Dual Specificity Phosphatase 10 (DUSP10)

Synonyms: MKP5, MKP-5, AI158871, 2610306G15Rik, DUSP10

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184694

Additional Information

Alternative name: DUSP10 / MKP5

Gene ID: 11221

Immunogen: CRILTPKLMGVETVV

Format: Affinity-purified

Description: All names and symbols: DUSP10, MKP5, dual specificity phosphatase 10, MKP-5, dual specificity phosphatase MKP-5, serine/threonine specific protein phosphatase. Official Symbol: DUSP10. RefSeq number(s): NP_009138.1, NP_653329.1. Gene ontology terms: protein phosphatase activity, JNK cascade, response to stress, protein amino acid dephosphorylation, cytoplasm, nucleus

Specificity: This antibody is expected to recognise both reported Human isofoms. The variants NP_653329.1 and NP_653330.1 represent identical protein. Human, Mouse, Rat, Dog, Cow 482 aa, 140 aa, 140 aa

Application Details

Application Notes: Western blot: Approx 53kDa band seen in Human Liver lysate. Recommended for use at 0.5-2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184694 staining (0.5µg/ml) of Human Liver lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Tanoue, Moriguchi, Nishida: "Molecular cloning and characterization of a novel dual specificity phosphatase, MKP-5." in: The Journal of biological chemistry, Vol. 274, Issue 28, pp. 19949-56, 1999 (PubMed).