BCL2-Antagonist/killer 1 (BAK1) antibody

Antigen: BCL2-Antagonist/killer 1 (BAK1)

Synonyms: BAK, CDN1, BCL2L7, MGC3887, BAK-LIKE, MGC117255

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184699

Additional Information

Alternative name: BAK1

Gene ID: 578, 12018 (mouse), 116502 (rat)

Immunogen: ASGQGPGPPRQE-C

Format: Affinity-purified

Description: All names and symbols: BAK1, BCL2-antagonist/killer 1, BAK, CDN1, BCL2L7, cell death inhibitor 1, apoptosis regulator BAK, Bcl-2 homologous antagonist/killer. Official Symbol: BAK1. RefSeq number(s): NP_001179. Gene ontology terms: apoptotic mitochondrial changes, induction of apoptosis, integral to membrane, apoptosis regulator activity

Specificity: Human, Mouse, Rat 211 aa

Application Details

Application Notes: Western blot: Approx 25kDa band seen in A431 cell lysate. Recommended for use at 0.5-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184699 staining (0.5µg/ml) of A431 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Derry, Ochs, Francke: "Isolation of a novel gene mutated in Wiskott-Aldrich syndrome." in: Cell, Vol. 78, Issue 4, pp. 635-44, 1994 (PubMed).