BAK1 antibody (AA 22-211)

Catalog No. ABIN3042342

Cited in 2+ publications. The Rabbit Polyclonal anti-BAK1 antibody (ABIN3042342) specifically detects BAK1 in WB, IHC, IF, FACS, ICC and IHC (fro). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for BAK1 antibody (AA 22-211) (ABIN3042342)

Target: BAK1 (BCL2-Antagonist/killer 1 (BAK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-BAK1 Antibody

Binding Specificity: AA 22-211

Purpose: Anti-BAK/BAK1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-BAK/BAK1 Antibody (ABIN3042342). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human BAK recombinant protein (Position: A22-S211). Human BAK shares 78.3 % amino acid (aa) sequence identity with mouse BAK.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-BAK1 antibody (ABIN3042342)

Zuo, Wu, Xu, Yang, Yan, Tan, Meng, Ying, Liu, Kang, Huang: "Minicircle-oriP-IFNγ: a novel targeted gene therapeutic system for EBV positive human nasopharyngeal carcinoma." in: PLoS ONE, Vol. 6, Issue 5, pp. e19407, (2011) (PubMed).

Tong, Zheng, Wang, Liu, Qian: "BAK overexpression mediates p53-independent apoptosis inducing effects on human gastric cancer cells." in: BMC cancer, Vol. 4, pp. 33, (2004) (PubMed).

Target Details for BAK1

Target: BAK1 (BCL2-Antagonist/killer 1 (BAK1))

Alternative Name: BAK1

Background:

Synonyms: Bcl-2 homologous antagonist/killer,Apoptosis regulator BAK,Bcl-2-like protein 7,Bcl2-L-7,BAK1,BAK, BCL2L7, CDN1,

Tissue Specificity: Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle.

Background: BAK, officially called Bcl2 antagonist killer, is a protein that in humans, encoded by the BAK gene. The BAK protein is a pro-apoptotic member of the Bcl-2 gene family which is involved in initiating apoptosis. BAK gene spans 7.6 kb and contains 6 exons. By Southern blot analysis of genomic DNA from human/rodent somatic cell hybrids, BAK gene is localized to chromosome 6. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.

Sequence Similarities: Belongs to the Bcl-2 family.

Molecular Weight: 25 kDa

Gene ID: 578

UniProt: Q16611

Pathways: Apoptosis, Steroid Hormone Mediated Signaling Pathway, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response