WNT4 antibody (Internal Region)

Catalog No. ABIN184715

The Goat Polyclonal anti-WNT4 antibody is suitable to detect WNT4 in samples from Human. It has been validated for WB, ELISA and IF.

Quick Overview for WNT4 antibody (Internal Region) (ABIN184715)

Target: WNT4 (Wingless-Type MMTV Integration Site Family, Member 4 (WNT4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This WNT4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-WNT4 Antibody

Binding Specificity: Internal Region

Purpose: WNT4

Sequence: SNWLYLAKLS SVGS

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SNWLYLAKLSSVGS, from the internal region of the protein sequence according to NP_110388.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 40+35 kDa band observed in lysates of cell line Jurkat (calculated MW of 39.1 kDa according to NP_110388.2). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature. Negative Control: U937

Peptide ELISA: antibody detection limit dilution 1:4000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the vesicles of HeLa and MCF7 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for WNT4

Target: WNT4 (Wingless-Type MMTV Integration Site Family, Member 4 (WNT4))

Alternative Name: WNT4

Background: WNT4, wingless-type MMTV integration site family, member 4, WNT-4, WNT-4 protein precursor, RP1-224A6.7, SERKAL, WNT-4 protein

Gene ID: 54361, 22417

NCBI Accession: NP_110388

Pathways: WNT Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cell-Cell Junction Organization, Tube Formation