ADAM Metallopeptidase Domain 17 (ADAM17) antibody

Antigen: ADAM Metallopeptidase Domain 17 (ADAM17)

Synonyms: CSVP, TACE, ADAM18, CD156B, MGC71942, Tace, CD156b, ADAM17, adam17, si:dkey-81b15.2

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184721

Additional Information

Alternative name: ADAM17 / TACE

Gene ID: 6868, 11491 (mouse)

Immunogen: LQRQNRVDSKETEC

Format: Affinity-purified

Description: All names and symbols: ADAM17, TACE, a disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme), CSVP, cSVP, CD156b, CD156b, MGC71942, TACE, cSVPADAM metallopeptidase domain 17, TNF-alpha converting enzyme, a disintegrin and metalloproteinase domain 17, a disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme), snake venom-like protease. Official Symbol: ADAM17. RefSeq number(s): NP_003174.3. Gene ontology terms: metallopeptidase, cell-cell signalling, integral plasma membrane protein, peripheral plasma membrane protein

Specificity: This antibody is expected to recognise isoform 1 (represented by NP_003174) and not isoform 2 (represented by NP_068604). Human, Mouse, Rat, Chinese Hamster

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 100kDa band observed in human testis lysates (calculated MW of 93.0kDa according to NP_003174.3). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184721 staining (0.2µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Black, Rauch, Kozlosky et al.: "A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells." in: Nature, Vol. 385, Issue 6618, pp. 729-33, 1997 (PubMed).