PEBP1 antibody (C-Term)

Catalog No. ABIN184729

This Goat Polyclonal antibody specifically detects PEBP1 in WB, ELISA and IHC. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for PEBP1 antibody (C-Term) (ABIN184729)

Target: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PEBP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-PEBP1 Antibody

Binding Specificity: C-Term

Purpose: PEBP1

Sequence: DYVPKLYEQL SGK

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DYVPKLYEQLSGK, from the C Terminus of the protein sequence according to NP_002558.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5 μg/mL.

Western Blot: Approx 20-22 kDa band observed in A549, HepG2 and Human Liver lysate (predicted MW of 22 kDa according to NP_002558.1). Recommended for use at 0.5-2 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-PEBP1 antibody (ABIN184729)

Weitzdörfer, Höger, Shim, Cekici, Pollak, Lubec: "Changes of hippocampal signaling protein levels during postnatal brain development in the rat." in: Hippocampus, Vol. 18, Issue 8, pp. 807-13, (2008) (PubMed).

Target Details for PEBP1

Target: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Alternative Name: PEBP1

Background: PEBP1, phosphatidylethanolamine binding protein 1, HCNP, PBP, PEBP, RKIP, prostatic binding protein, RAF kinase inhibitor protein, Raf kinase inhibitory protein, hippocampal cholinergic neurostimulating peptide

Gene ID: 5037, 23980

NCBI Accession: NP_002558

Pathways: Feeding Behaviour