Phosphatidylethanolamine Binding Protein 1 (PEBP1) antibody

Antigen: Phosphatidylethanolamine Binding Protein 1 (PEBP1)

Synonyms: PBP, HCNP, PEBP, RKIP, pbp, hcnp, pebp, rkip, Pbp, Pbp1, Pbpr, MGC102065, MGC103131, Rkip, MGC82659, MGC130722, PEBP1, DKFZp459B183

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184729

Additional Information

Alternative name: PEBP1

Gene ID: 5037, 23980 (mouse)

Immunogen: C-DYVPKLYEQLSGK

Format: Affinity-purified

Description: All names and symbols: PEBP1, phosphatidylethanolamine binding protein 1, HCNP, PBP, PEBP, RKIP , prostatic binding protein, RAF kinase inhibitor protein. Official Symbol: PEBP1. RefSeq number(s): NP_002558.1. Gene ontology terms: phosphatidylethanolamine binding activity

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 22kDa band observed in lysates of Human Kidney fibroblast HEK293, Human lung carcinoma A549 and Human Hepatoblastoma HepG2 cell lines (calculated MW of 21.1kDa according to NP_002558.1). Recommended concentration: 0.01-0.03µg/ml. A customer has reported that this antibody also works well by western blot in Rat Hippocampus.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184729 staining (0.01µg/ml) of A549 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Yeung, Seitz, Li et al.: "Suppression of Raf-1 kinase activity and MAP kinase signalling by RKIP." in: Nature, Vol. 401, Issue 6749, pp. 173-7, 1999 (PubMed).