Growth Factor Receptor-Bound Protein 7 (GRB7) (N-Term) antibody

Antigen: Growth Factor Receptor-Bound Protein 7 (GRB7)

Synonyms: KIAA4028, MGC78181, mKIAA4028, MGC93307, GRB7, MGC138032

Epitope: N-Term

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184737

Additional Information

Alternative name: GRB7 (N Terminus)

Gene ID: 2886, 14786 (mouse)

Immunogen: ELDLSPPHLSSSPE-C

Format: Affinity-purified

Description: All names and symbols: GRB7, growth factor receptor-bound protein 7. Official Symbol: GRB7. RefSeq number(s): NP_005301. Gene ontology terms: SH3/SH2 adaptor protein activity, EGF receptor signaling pathway, oncogenesis

Specificity: This antibody recognises the longer isoform of this protein in A431 lysate. Human 532 aa

Application Details

Application Notes: Western blot: Approx 60-65kDa band seen in A431 lysate. Recommended for use at 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184737 staining (2µg/ml) of A431 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Margolis, Silvennoinen, Comoglio et al.: "High-efficiency expression/cloning of epidermal growth factor-receptor-binding proteins with Src homology 2 domains." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 19, pp. 8894-8, 1992 (PubMed).