GRB7 antibody (Middle Region)

Catalog No. ABIN5518677

This Rabbit Polyclonal antibody specifically detects GRB7 in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.It has been mentioned in 1 publication

Quick Overview for GRB7 antibody (Middle Region) (ABIN5518677)

Target: GRB7 (Growth Factor Receptor-Bound Protein 7 (GRB7))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRB7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GRB7 Antibody

Binding Specificity: AA 124-141, Middle Region

Purpose: Anti-GRB7 Antibody

Sequence: ARHVCEMLVQ RAHALSDE

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-GRB7 Antibody (ABIN5518677). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human GRB7, identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-GRB7 antibody (ABIN5518677)

Zeng, Yang, Hu, Liu, Yang, Ran, Li, Li, Yu: "Grb7 gene amplification and protein expression by FISH and IHC in ovarian cancer." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 11296-304, (2016) (PubMed).

Target Details for GRB7

Target: GRB7 (Growth Factor Receptor-Bound Protein 7 (GRB7))

Alternative Name: GRB7

Background:

Synonyms: Growth factor receptor-bound protein 7,B47,Epidermal growth factor receptor GRB-7,GRB7 adapter protein,GRB7,

Background: GRB7, growth factor receptor-bound protein 7, is a protein which in humans is encoded by the GRB7 gene. The product of this gene belongs to a small family of adaptor proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. The protein plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Alternative splicing results in multiple transcript variants encoding different isoforms, although the full-length natures of only two of the variants have been determined to date. GRB7 is an SH2-domain adaptor protein that binds to receptor tyrosine kinases and provides the intra-cellular link to the Ras proto-oncogene. Human GRB7 is located on the long arm of chromosome 17, next to the ERBB2 (alias HER2/neu) proto-oncogene. These two genes are commonly co-amplified (present in excess copies) in breast cancers. GRB7 thought to be involved in migration, is well known to be over-expressed in testicular germ cell tumors, esophageal cancers, and gastric cancers.

Sequence Similarities: Belongs to the GRB7/10/14 family.

Molecular Weight: 60 kDa

Gene ID: 2886

UniProt: Q14451

Pathways: EGFR Signaling Pathway, Ribonucleoprotein Complex Subunit Organization