GRB7 antibody (Middle Region)

Catalog No. ABIN5518677

Product Details anti-GRB7 Antibody

Target: GRB7 (Growth Factor Receptor-Bound Protein 7 (GRB7))

Binding Specificity: AA 124-141, Middle Region

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRB7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Growth factor receptor-bound protein 7(GRB7) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: ARHVCEMLVQ RAHALSDE

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Growth factor receptor-bound protein 7(GRB7) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: growth factor receptor-bound protein 7
Protein Name: Growth factor receptor-bound protein 7

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human GRB7(124-141aa ARHVCEMLVQRAHALSDE), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-GRB7 antibody (ABIN5518677)

Zeng, Yang, Hu, Liu, Yang, Ran, Li, Li, Yu: "Grb7 gene amplification and protein expression by FISH and IHC in ovarian cancer." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 11296-304, (2016) (PubMed).

Target Details for GRB7

Target: GRB7 (Growth Factor Receptor-Bound Protein 7 (GRB7))

Alternative Name: GRB7 (GRB7 Products)

Synonyms: GRB7 antibody, mKIAA4028 antibody, growth factor receptor bound protein 7 antibody, GRB7 antibody, Grb7 antibody

Background: GRB7, growth factor receptor-bound protein 7, is a protein which in humans is encoded by the GRB7 gene. The product of this gene belongs to a small family of adaptor proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. The protein plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Alternative splicing results in multiple transcript variants encoding different isoforms, although the full-length natures of only two of the variants have been determined to date. GRB7 is an SH2-domain adaptor protein that binds to receptor tyrosine kinases and provides the intra-cellular direct link to the Ras proto-oncogene. Human GRB7 is located on the long arm of chromosome 17, next to the ERBB2 (alias HER2/neu) proto-oncogene. These two genes are commonly co-amplified (present in excess copies) in breast cancers. GRB7 thought to be involved in migration, is well known to be over-expressed in testicular germ cell tumors, esophageal cancers, and gastric cancers.

Synonyms: B47 antibody|Epidermal growth factor receptor GRB 7 antibody|Epidermal growth factor receptor GRB-7 antibody|GRB7 adapter protein antibody|GRB7 antibody|GRB7_HUMAN antibody|Growth factor receptor bound protein 7 antibody|Growth factor receptor-bound protein 7 antibody|OTTHUMP00000164349 antibody|OTTHUMP00000164350 antibody|OTTHUMP00000164351 antibody|OTTHUMP00000164352 antibody

Gene ID: 2886

UniProt: Q14451

Pathways: EGFR Signaling Pathway, Ribonucleoprotein Complex Subunit Organization