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anti-AIF1 / IBA1 (isoform 1 and 3) antibody

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Antigen: AIF1 / IBA1 (isoform 1 and 3)
Clonality: Polyclonal
Application: ELISA, Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Reactivity: Human, Rat
Host: Goat
1 reference available 1 reference available
Quantity: 100µg (100µg specific antibody in 20...)
Price: 498,75 $   (Plus shipping costs $25 and VAT, Invoice in EUR)
Order number: ABIN184738
Availability: Ships within 5 Business days
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Additional Information  » anti-AIF1 / IBA1 (isoform 1 and 3) antibody

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Antigen AIF1 / IBA1 (isoform 1 and 3)
Gene ID 199 , 11629 (mouse) , 29427 (rat)
Immunogen Peptide with sequence C-TGPPAKKAISELP, from the C Terminus of the protein sequence according to NP_116573.1, NP_001614.3
Reactivity Human, Rat
Clonality Polyclonal
Format Aff - Purified
Description All names and symbols: AIF1, IBA1, allograft inflammatory factor 1, AIF-1, IRT-1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule, Daintain, DADB-70P7.8. Official Symbol: AIF1. RefSeq number(s): NP_116573.1, NP_001614.3. Gene ontology terms: cell cycle arrest, negative regulation of cell proliferation, response to stress, inflammatory response, nucleus This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3)..
Host Goat
Specificity Expected from sequence similarity: Human, Mouse, Rat, Pig. Important Comments: This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).

Application Details  » anti-AIF1 / IBA1 (isoform 1 and 3) antibody

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Application ELISA, Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
» Click here to view 2 available protocols!
Application Notes Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 17kDa band observed in Rat Brain lysates (calculated MW of 16.7kDa according to Human NP_001614.3 and of 16.8kDa according to Rat NP_058892.1). Recommended concentration: 1-3µg/ml. IHC-Fr: 1/500 - 1/1000. Freeze tissue in isopentane, postfix sections in PFA 2% for 1 hour. IHC-P: 1/100. Perform heat mediated antigen retrieval via the microwave method.
Concentration 100µg specific antibody in 200µl
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Buffer 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.
Research Area Cell Cycle, Immunology, Neurology
Restrictions For Research Use only

Publications  » anti-AIF1 / IBA1 (isoform 1 and 3) antibody

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Publications Autieri, Agrawal: "IRT-1, a novel interferon-gamma-responsive transcript encoding a growth-suppressing basic leucine zipper protein." In: The Journal of biological chemistry , Vol. 273, Issue 24, pp. 14731-7, 1998 (PubMed)