Allograft Inflammatory Factor 1 (AIF1) (isoform 1 and 3) antibody

Antigen: Allograft Inflammatory Factor 1 (AIF1)

Synonyms: IBA1, AIF-1, IRT-1, G1, Iba1, AI607846, D17H6S50E, iba1, Bart1, mrf-1, fa04b11, wu:fa04b11, AIF1, AIF, aif1

Epitope: isoform 1 and 3

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN184738

Additional Information

Alternative name: AIF1 / IBA1 (isoform 1 and 3)

Gene ID: 199, 11629 (mouse), 29427 (rat)

Immunogen: Peptide with sequence C-TGPPAKKAISELP, from the C Terminus of the protein sequence according to NP_116573.1, NP_001614.3

Format: Affinity-purified

Description: All names and symbols: AIF1, IBA1, allograft inflammatory factor 1, AIF-1, IRT-1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule, Daintain, DADB-70P7.8. Official Symbol: AIF1. RefSeq number(s): NP_116573.1, NP_001614.3. Gene ontology terms: cell cycle arrest, negative regulation of cell proliferation, response to stress, inflammatory response, nucleus This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3)..

Specificity: Expected from sequence similarity: Human, Mouse, Rat, Pig. Important Comments: This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 17kDa band observed in Rat Brain lysates (calculated MW of 16.7kDa according to Human NP_001614.3 and of 16.8kDa according to Rat NP_058892.1). Recommended concentration: 1-3µg/ml. IHC-Fr: 1/500 - 1/1000. Freeze tissue in isopentane, postfix sections in PFA 2% for 1 hour. IHC-P: 1/100. Perform heat mediated antigen retrieval via the microwave method.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.

Research Area: Cell Cycle, Immunology, Neurology

Restrictions: For Research Use only

Images

ABIN184738 (0.5µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Autieri, Agrawal: "IRT-1, a novel interferon-gamma-responsive transcript encoding a growth-suppressing basic leucine zipper protein." in: The Journal of biological chemistry, Vol. 273, Issue 24, pp. 14731-7, 1998 (PubMed).