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Allograft Inflammatory Factor 1 (AIF1) (isoform 1 and 3) antibody

Antigen

Allograft Inflammatory Factor 1 (AIF1)

Synonyms IBA1, AIF-1, IRT-1, G1, Iba1, AI607846, D17H6S50E, iba1, Bart1, mrf-1, fa04b11, wu:fa04b11, AIF1, AIF, aif1
Epitope
Alternatives

isoform 1 and 3

Clonality Polyclonal
Host
Reactivity
Alternatives

Human, Rat (Rattus)

Application
Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
1 reference available
Catalog no. ABIN184738
Quantity 100µg  (100µg specific antibody in 200µl)  (Variants)
Price 501.80 $   Plus shipping costs $35.00
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Additional Information

Alternative name AIF1 / IBA1 (isoform 1 and 3)
Gene ID 199, 11629 (mouse), 29427 (rat)
Immunogen Peptide with sequence C-TGPPAKKAISELP, from the C Terminus of the protein sequence according to NP_116573.1, NP_001614.3
Format Affinity-purified
Description All names and symbols: AIF1, IBA1, allograft inflammatory factor 1, AIF-1, IRT-1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule, Daintain, DADB-70P7.8. Official Symbol: AIF1. RefSeq number(s): NP_116573.1, NP_001614.3. Gene ontology terms: cell cycle arrest, negative regulation of cell proliferation, response to stress, inflammatory response, nucleus This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3)..
Specificity Expected from sequence similarity: Human, Mouse, Rat, Pig. Important Comments: This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).

Application Details

Application Notes Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 17kDa band observed in Rat Brain lysates (calculated MW of 16.7kDa according to Human NP_001614.3 and of 16.8kDa according to Rat NP_058892.1). Recommended concentration: 1-3µg/ml. IHC-Fr: 1/500 - 1/1000. Freeze tissue in isopentane, postfix sections in PFA 2% for 1 hour. IHC-P: 1/100. Perform heat mediated antigen retrieval via the microwave method.
Concentration 100µg specific antibody in 200µl
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Buffer 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.
Research Area Cell Cycle, Immunology, Neurology
Restrictions For Research Use only

Publications

Publications Autieri, Agrawal: "IRT-1, a novel interferon-gamma-responsive transcript encoding a growth-suppressing basic leucine zipper protein." in: The Journal of biological chemistry, Vol. 273, Issue 24, pp. 14731-7, 1998 (PubMed).

Alternatives

Alternatives for antigen "Allograft Inflammatory Factor 1 (AIF1)", type "Antibodies"
Hosts Rabbit (20), Mouse (8), Goat (7)
Reactivities Human (30), Rat (Rattus) (13), Mouse (Murine) (10), Dog (Canine) (3), Cow (Bovine) (2)
Applications Western Blotting (WB) (29), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Enzyme Immunoassay (EIA) (6), ELISA (4), Immunofluorescence (IF) (4), ELISA (Detection) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Immunoprecipitation (IP) (2), Flow Cytometry (FACS) (1), Immunohistochemistry (IHC) (1)
Conjugates Biotin (1), FITC (1)
Epitopes isoform 1 and 3 (2), isoform 3 (2)