Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2) antibody

Antigen: Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2)

Synonyms: ARC34, PRO2446, p34-Arc, PNAS-139, arc34, p34-arc, pro2446, MGC69338, pnas-139, ARPC2, MGC126898, Arpc2, Arc-p34, DKFZp459D1927, MGC154528

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN184748

Additional Information

Alternative name: ARPC2

Gene ID: 10109, 76709 (mouse)

Immunogen: Peptide with sequence C-EMKTITGKTFSSR, from the C Terminus of the protein sequence according to NP_005722.1, NP_690601.1

Format: Affinity-purified

Description: All names and symbols: ARPC2, actin related protein 2/3 complex, subunit 2, 34kDa, ARC34, PRO2446, p34-Arc, PNAS-139, ARP2/3 protein compex subunit 34, actin related protein 2/3 complex subunit 2. Official Symbol: ARPC2. RefSeq number(s): NP_005722.1, NP_690601.1. Gene ontology terms: cell motility, actin cytoskeleton, Arp2/3 protein complex, cytoskeletal structural protein The reported variants NP_005722.1 and NP_690601.1 represent identical protein..

Specificity: Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 33-34kDa band observed in Hela and Human Skeletal Muscle lysates (predicted MW of 34kDa according to NP_005722). Recommended for use at 0.1-0.5µg/ml. Immunohistochemistry: In paraffin embedded Human Spleen shows cytoplasm staining in some splenocytes in and around a T-cell area. Recommended concentration, 3-5µg/ml.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.

Research Area: Signaling, Cytoskeleton, Microfilaments

Restrictions: For Research Use only

Publications

Publications: Welch, DePace, Verma et al.: "The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly." in: The Journal of cell biology, Vol. 138, Issue 2, pp. 375-84, 1997 (PubMed).